CONTROL OF AXIAL MUSCLE DEVELOPMENT

控制轴向肌发育

• 批准号: 2838789
• 负责人: KATHRYN W TOSNEY
• 金额: $ 19.96万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-21 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2838789
• 关键词: cell differentiation; cell type; chick embryo; in situ hybridization; mesenchyme; muscle cells; myoblasts; myogenesis; myotubes; quail; regulatory gene; scanning electron microscopy; transmission electron microscopy

Determination, differentiation, and patterned morphogenesis are central processes during development, but the mechanisms controlling them have yet to be satisfactorily identified. These processes are accessible to analysis in a simple vertebrate system, the development of axial muscle from the epithelial dermatome in the chicken embryo. Pilot studies suggest that the dermatome generates two cell phenotypes under a tight spatial control. Furthermore, this generation pattern can be altered experimentally. A simple surgery causes the dermacyte-forming population to form myocytes instead. Moreover, the dermatome gives rise to axial muscles that manifest different fates at limb and thoracic levels. For instance, preliminary results show that programmed death of dermatome prevents development of lateral axial muscles at limb levels and suggest that this death is induced by an interaction with limb tissue. Proposed studies focus on the spatial control of cell generation in this system and the interactions controlling cell generation patterns and muscle fate. Studies in five aims will 1) finely map the fate of dermatome cells and characterize the development of muscles derived from dermatome, 2) assess the pluripotency of spatially defined dermatomal populations, 3) define interactions that control the patterned generation of myocytes and dermacytes, 4) define interactions that control the segment-specific fates of axial muscles and 5) assess the relevance of putative regulatory genes by defining expression patterns and using experimental manipulations. The approaches combine anatomical methods, fate-mapping strategies and embryonic surgeries. This work will help elucidate mechanisms that control the patterned generation of specific cell types and the morphogenesis of muscles, processes that are fundamental to normal human development.

确定，分化和图案形态发生是中心 开发过程中的过程，但是控制它们的机制 令人满意地确定。 这些过程可访问 在简单的脊椎动物系统中分析轴向肌肉的发展 从鸡肉胚胎中的上皮皮肤病。 试点研究 建议皮肤病在紧密的情况下产生两个细胞表型 空间控制。 此外，这一代模式可以改变 实验。 简单的手术会导致形成皮肤的人群 改为形成心肌细胞。 此外，皮肤组会产生轴向 在肢体和胸腔水平上表现出不同命运的肌肉。 为了 实例，初步结果表明皮肤病的编程死亡 防止肢体水平的侧轴向肌肉的发育，并建议 这种死亡是由与肢体组织的相互作用引起的。 拟议的研究重点是对细胞产生的空间控制 系统和控制细胞生成模式的相互作用以及 肌肉命运。 五个目标的研究将为1）精心绘制 真皮细胞并表征从 皮肤病组，2）评估空间定义的皮肤病的多能性 人群，3）定义控制图案生成的相互作用 肌细胞和皮肤，4）定义控制的相互作用 轴向肌肉的特定阶段命运，5）评估相关性 通过定义表达模式并使用的推定调节基因 实验操作。 这些方法结合了解剖方法， 命运策略和胚胎手术。 这项工作将有所帮助 阐明控制特定生成的图案生成的机制 细胞类型和肌肉的形态发生，过程 正常人类发展的基础。