LOCUS COERULEUS AND RAPHE ACTIVITY IN SLEEP AND WAKING

睡眠和清醒时的蓝斑和中缝活动

• 批准号: 6056637
• 负责人: MONICA M EILAND
• 金额: $ 2.02万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6056637
• 关键词: Chelonia; REM sleep; acetylcholine; brain electrical activity; cell population study; dorsal raphe nucleus; electroencephalography; electromyography; immunocytochemistry; locus coeruleus; serotonin; sleep; tegmentum; wakefulness

DESCRIPTION (adapted from applicant's abstract): Phylogenetic comparisons between mammals and reptiles could provide important clues to the function of sleep. Features of sleep, aminergic activity and cholinergic activity common to the reptilian, avian, and mammalian lines are likely to have been present in the stem reptiles. In mammals, the adrenergic cells of the locus coeruleus (LC) and the serotonergic cells of the raphe are tonically active in waking, decrease discharge in non-REM sleep, and are silent in REM sleep. Mammals also possess two cholinergic cell populations that produce the tonic and phasic components of waking and REM. The function or evolutionary history of these discharge profiles is not known. Narcolepsy, depression and REM sleep behavior disorder may result from disturbance of these discharging profiles. Though these immunohistochemically-defined populations are also found in reptiles, their activity has not been recorded in unanesthetized reptiles. The investigators have developed methods for recording brainstem units from a reptile, the common box turtle, Terrapene Carolina, during sleep and waking behaviors. They will identify and characterize the locus coeruleus, raphe, and cholinergic cell groups of the pedunculopontine and laterodorsal tegmentum immunohistochemically and electrophysiologically. They will compare these cells' state-dependent unit activity with that of mammals in order to provide insights into mthe evolutionary significance of sleep.

描述（改编自申请人的摘要）：系统发育比较 在哺乳动物和爬行动物之间可以为功能提供重要的线索 睡眠。 睡眠，氨基活性和胆碱能活性的特征 爬行动物，禽和哺乳动物的共同点可能是 存在于茎爬行动物中。 在哺乳动物中，该基因座的肾上腺素能细胞 Raphe的Coeruleus（LC）和血清素能细胞是有效的 在醒来时，减少非REM睡眠的排出，并在REM睡眠中保持沉默。 哺乳动物还拥有两个产生补品的胆碱能细胞群体 以及醒来和雷姆的阶段组成部分。 功能或进化 这些排放曲线的历史尚不清楚。 发肠病毒，抑郁症 REM睡眠行为障碍可能是由于这些干扰而引起的 放电轮廓。 尽管这些免疫组织化学定义 种群也发现了爬行动物，尚未记录其活动 在一体式爬行动物中。 调查人员已经开发了 从爬行动物，公共盒龟，terrapene记录脑干单元 卡罗来纳州，在睡眠和醒来的行为中。 他们将识别并 表征层层层，raphe和胆碱能细胞基团 pedunculopontine和Laterodorsal tementum免疫组织化学和 电生理学。 他们将比较这些单元的状态依赖性单元 哺乳动物的活动以提供对Mthe的见解 睡眠的进化意义。