CYTOKINE SECRETION IN MIXED CELL POPULATIONS

混合细胞群中的细胞因子分泌

• 批准号: 2715738
• 负责人: YEVGENYA AKSELBAND
• 金额: $ 10万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2715738
• 关键词: animal tissue; cell population study; cytokine; differentiation antigens; flow cytometry; gel; immunofluorescence technique; interferon gamma; interleukin 6; lymphocyte; method development; microcapsule; mixed tissue /cell culture; secretion

The aim of this project is to develop an immunofluorescent assay system capable of quantitating the secretion of cytokines by individual cells in mixed cell populations by encapsulating individual cells in agarose gel microdrops (GMD) and then measuring secretion of cytokine molecules after appropriate stimulation. The combination of immunofluorescent staining of secreted cytokines and cell surface labeling techniques will permit simultaneous determination of the phenotype of cytokine-secreting cells by using two or three-color flow cytometric analysis. the availability of this assay system could become an important tool for basic research, clinical diagnostic and industrial applications. PROPOSED COMMERCIAL APPLICATION: The majority of commercialized biotechnology products are cytokines. In addition the cytokine research product market is in excess of $500 M. New assay formats for quantitating cytokine secretion at the single cell level would also impact clinical diagnostic research.

该项目的目的是开发免疫荧光测定系统 能够定量单个细胞在 通过将单个细胞封装在琼脂糖凝胶中的混合细胞群体 微晶体（GMD），然后测量细胞因子分子的分泌。 适当的刺激。免疫荧光染色的组合 分泌的细胞因子和细胞表面标记技术将允许 同时测定细胞因子分泌细胞的表型 使用两个或三色流式细胞仪分析。可用性 该测定系统可能成为基础研究的重要工具， 临床诊断和工业应用。 拟议的商业应用： 大多数商业化生物技术产品都是细胞因子。在 补充细胞因子研究产品市场超过500美元。 分析格式用于定量单细胞水平的细胞因子分泌 还会影响临床诊断研究。