NOVEL METHOD FOR FUNGAL SUSCEPTIBILITY TESTING

真菌敏感性测试的新方法

• 批准号: 2647337
• 负责人: YEVGENYA AKSELBAND
• 金额: $ 45.9万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2647337
• 关键词: Fungi; antifungal agents; cell line; drug resistance; flow cytometry; gel; genetic strain; method development; microcapsule; microorganism growth

DESCRIPTION: (Adapted from the Applicant's Abstract): This is a Phase II SBIR proposal to detect fungal growth and anti-fungal drug sensitivity using One Cell System's gel microdrop encapsulation technology (GMD) combined with flow cytometry (FACS) analysis and cell sorting. Phase I studies will be expanded to evaluate additional potentially pathogenic fungi. Results obtained using model cell lines will further be compared with results using clinical isolates of several pathogenic fungal strains. In addition, GMD will be integrated with nucleic acid probe identification and amplification models. This rapid and sensitive assay will impact clinical diagnostics and drug discovery by improving antifungal susceptibility testing and permitting recovery of microorganisms of interest for subsequent cloning or gene sequence analysis.

描述：（根据申请人的摘要进行了改编）：这是II阶段 SBIR提案以检测真菌生长和抗真菌药物敏感性 使用一个单元系统的凝胶微旋转封装技术（GMD） 结合流式细胞仪（FACS）分析和细胞分选。第一阶段 研究将扩展以评估其他潜在的致病性 真菌。使用模型细胞系获得的结果将进一步比较 结果使用几种致病真菌菌株的临床分离株。 此外，GMD将与核酸探针鉴定集成 和放大模型。 这种快速而敏感的测定将影响 临床诊断和药物发现通过改善抗真菌 敏感性测试并允许恢复微生物 随后的克隆或基因序列分析的兴趣。