IN SITU PHOTO CROSSLINKED COLLAGEN/GF FOR BONE REPAIR

用于骨修复的原位照片交联胶原蛋白/GF

• 批准号: 2538809
• 负责人: DAVID L CLAPPER
• 金额: $ 10万
• 依托单位: SURMODICS, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-25 至 2000-01-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2538809
• 关键词: biomaterial development /preparation; biomaterial evaluation; bone fracture; bone morphogenetic proteins; bone regeneration; chemical synthesis; collagen; crosslink; cytotoxicity; dogs; gel; growth factor; histology; laboratory rat; orthopedics; osteogenesis; pathologic bone resorption; photochemistry; radiography

Several dental and orthopedic conditions would benefit from an improved procedure for promoting bone regeneration or augmentation. The goal of this project is to develop a biodegradable osteoinductive preparation that will promote spatially-controlled bone regeneration in vivo. The preparation will consist of an osteoinductive growth factor and a collagen carrier, with the collagen carrier being derivatized by addition of photoactivatible groups. The preparation will be a viscous liquid when placed in the bone defect and will both solidify and bond to the remaining bone after being illuminated by a standard dental composite curing lamp. The collagen carrier will be a combination of insoluble particles of type I collagen (which will biodegrade slowly and provide an osteoconductive surface for migration and differentiation of osteoblasts) and photoderivatized type I collagen (which will crosslink to solidify the preparation). Three different photochemisty approaches will be compared for producing collagen gels, with the different variants being evaluated in vitro for strength and cytotoxicity, in rat implants as an inexpensive screening assay for bone formation in vivo, and in surgically-created bone defects in dogs as an efficacy test for bone regeneration around the roots of teeth. An osteoinductive collagen/GF preparation that can be crosslinked in vivo is expected to be used for several applications, including: 1) restoration of bone loss around the roots of teeth or dental implants, 2) augmentation of facial bones, 3) filling of defects in compound fractures, and 4) stabilization of vertebrae after spinal fusion. PROPOSED COMMERCIAL APPLICATION: An osteoinductive collagen/growth factor preparation that can be crosslinked in vivo should have broad application in several areas, including: 1) restoration of bone loss around the roots of teeth or dental implants, 2) augmentation of facial bones, 3) filling of defects in compound fractures, and 4) stabilization of vertebrae after spinal fusion.

几种牙齿和骨科条件将受益于改善 促进骨再生或增强的程序。 目标 该项目是为了开发可生物降解的骨诱导制剂 这将促进体内空间控制的骨再生。 这 制备将由骨诱导生长因子和A组成 胶原蛋白载体，胶原蛋白载体被衍生 添加光活性组。 准备工作将是一种粘性 液体放置在骨骼缺陷中，并将凝固和键合 被标准牙齿照亮后，剩余的骨头 复合固化灯。 胶原蛋白载体将是 I型胶原蛋白的不溶性颗粒（该胶原蛋白会缓慢生物降解，并且 提供了迁移和分化的骨电导表面 成骨细胞）和光重新生化的I型胶原蛋白（它将交叉链接 巩固准备工作）。 三种不同的光化学方法 将与产生胶原蛋白凝胶进行比较，不同 大鼠的强度和细胞毒性的体外评估变体 植入物作为体内骨形成的廉价筛查测定法， 并在狗的手术创建的骨缺损中作为疗效测试 牙齿根部周围的骨骼再生。 骨诱导 可以在体内交联的胶原蛋白/GF制备 用于多种应用，包括：1）恢复骨质流失 在牙齿或牙科植入物的根周围，2）面部 骨头，3）填充复合骨折中的缺陷，4）稳定 脊柱融合后的椎骨。 拟议的商业应用： 可以是骨诱导胶原蛋白/生长因子制备 交联体体内应在多个领域具有广泛的应用， 包括：1）牙齿根部周围恢复骨质流失或 牙科植入物，2）增强面部骨骼，3）填充缺陷 在复合骨折中，4）脊柱后椎骨稳定 融合。