FIBER OPTIC FLUORESCENCE DETECTOR FOR PHOTOSENSITIZER

光敏剂光纤荧光检测器

基本信息

批准号：
2715013
负责人：
GREGORY C BURKE
金额：
$ 9.76万
依托单位：
AURORA OPTICS, INC.
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-09-30 至 2000-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2715013
关键词：
biomedical equipment development biopsy fiber optics fluorescent dye /probe ionophores laboratory mouse measurement method development neoplasm /cancer photoradiation therapy phantom model photosensitizing agents

项目摘要

A technique will be developed to measure the concentration of a fluorophore in vivo, from detection of the fluorescence to reflected light ratio using a specialized fiber optic bundle which limits the probed tissue volume. This technique will allow real-time in vivo optical biopsy of any fluorophores from a tissue. By limiting the probe (fiber optic) area on the tissue to smaller than the typical scattering length, the detected signal is insensitive to the tissue's natural absorption characteristics, and is proportional to only the fluorophore concentration. Then by dividing the fluorescent light by the reflected light, the variations in scattering coefficient are eliminated from the measurement. For example, the fluorescence signal from dark and light pigmented skin should be equal, for equal concentrations of fluorophore. In photodynamic therapy, it can be used to measure the uptake of photosensitizer within tissues, and may eventually replace time consuming tissue extraction methods which are currently used. A special fiber optic bundle will be created which samples a number of small spots on the tissue, and integrates the signals into a single measurement. This process retains the insensitivity to the tissue type while allowing lower light intensities to be used. PROPOSED COMMERCIAL APPLICATIONS: The fiber optic based system outlined in this proposal would be an extremely useful tool for all clinics involved in photodynamic therapy research and clinical trials. Essentially all of these labs need to quantitate the photosensitizer uptake in tissue for accurate treatment planning. Current methods are time consuming and expensive. Beyond photodynamic therapy, this device would also have potential to provide pharmacokinetic studies of fluorescent tracer drugs injected in vivo.

将开发一种测量A的浓度的技术荧光团在体内，从荧光检测到反射使用专门的光纤束的光比探测的组织体积。该技术将允许实时体内光学来自组织的任何荧光团的活检。通过限制探针（纤维组织上的面积比典型的散射长度小，检测到的信号对组织的自然吸收不敏感特征，仅与荧光团成正比专注。然后将荧光除以反射光线，从散射系数中的变化从测量。例如，来自黑暗和光线的荧光信号色素皮肤应等于相等的荧光团。在光动力疗法中，它可用于测量纸巾中的光敏剂，最终可能取代耗时当前使用的组织提取方法。特殊光纤将创建捆绑包，以在组织，并将信号整合到单个测量中。这过程保持对组织类型的不敏感性，同时允许较低要使用的光强度。拟议的商业应用：该提案中概述的基于光纤的系统将是对于所有参与光动力疗法的诊所的工具非常有用研究和临床试验。本质上，所有这些实验室都需要量化组织中的光敏剂吸收以进行准确治疗规划。当前的方法耗时且昂贵。超过光动力疗法，该设备也有可能提供在体内注射荧光示踪剂药物的药代动力学研究。