TYPE X COLLAGEN IN THE AGING SKELETON

老化骨骼中的 X 型胶原蛋白

• 批准号: 2632678
• 负责人: THOMAS M SCHMID
• 金额: $ 6.8万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2632678
• 关键词: age difference; aging; apoptosis; articular cartilage; calcium ion; cell differentiation; chondrocytes; collagen; human tissue; immunocytochemistry; nitric oxide; osteoarthritis; protein biosynthesis; western blottings

Type X collagen is a short chain collagen which is synthesized almost exclusively by the most mature chondrocytes in cartilage near the calcification front at the cartilage-bone junction. It is a specific biosynthetic marker for the terminal stage of chondrocyte differentiation during endochondral bone formation. This molecule is not synthesized by other cells nor do immature chondrocytes make it until they have enlarged, that is reached a hypertrophic stage. In the growth plate this is the final stage of the chondrocyte life cycle when these cells initiate the mineralization process and then die by apoptosis. The majority of the information on the association of type X collagen with cartilage cell differentiation comes from embryonic studies on nonhuman cartilage. Little is known about the appearance of type X collagen in the aging human skeleton. Several laboratories have reported the appearance of type X collagen in osteoarthritic cartilage, in matrix vesicles of osteoarthritic cartilage, in the formation of bony spurs (osteophytes) and with the movement of the mineralization front (tidemark). The goal of these studies will be to evaluate the prevalence of type X collagen during the normal aging of adult human cartilage. A second goal is to evaluate the potential of human articular chondrocytes isolated from tissue donors of different ages to synthesize type X collagen in response to elevated concentrations of calcium ions and nitric oxide. An understanding of the chondrocyte differentiation process in the aging skeleton will provide a foundation for therapeutic intervention in disorders like OA which are most prevalent in the elderly.

X型胶原蛋白是一种短链胶原蛋白，几乎合成 完全由软骨中最成熟的软骨细胞在附近 软骨骨交界处的钙化前端。 这是一个特定的 软骨细胞末端的生物合成标记 内侧软骨骨形成过程中的分化。这个分子不是 由其他细胞合成，也不是未成熟的软骨细胞直到 它们已经扩大，达到了肥厚阶段。 在 生长板这是软骨细胞生命周期的最后阶段 这些细胞启动矿化过程，然后死亡 凋亡。 大多数有关类型关联的信息 带有软骨细胞分化的X胶原蛋白来自胚胎 关于非人类软骨的研究。 关于外观知之甚少 老化的人骨骼中的X型胶原蛋白。 几个实验室有 报道了X型胶原蛋白在骨关节炎软骨中的出现， 在骨关节炎软骨的基质囊泡中 马刺（骨植物）和矿化前部的运动 （Tidemark）。 这些研究的目的是评估 成年人正常老化期间X型胶原蛋白的患病率 软骨。 第二个目标是评估人的潜力 从不同年龄的组织供体分离到的关节软骨细胞 合成X型 胶原蛋白响应钙离子浓度升高和 一氧化氮。 对软骨细胞分化的理解 老化骨骼的过程将为治疗提供基础 干预诸如OA之类的疾病，最普遍 老年。