ANTIOXIDATIVE THERAPY FOR CARDIAC ARREST--AN NMR STUDY

心脏骤停的抗氧化治疗——核磁共振研究

• 批准号: 2771212
• 负责人: SERGUEI M LIACHENKO
• 金额: $ 4.53万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-14 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2771212
• 关键词: acidity /alkalinity; adenosine triphosphate; antioxidants; bioenergetics; brain mapping; brain metabolism; cardiopulmonary resuscitation; creatine phosphate; disease /disorder model; heart arrest; histopathology; hypoxia; ischemia; laboratory rat; lactates; lipid peroxides; nerve injury; neurophysiology; nuclear magnetic resonance spectroscopy

In the United States, about 500,000 sudden cardiac deaths are registered each year. Global brain ischemia is the major pathophysiological event that limits the time available for patient rescue to 5-10 min. This study investigates the effects of antioxidative therapy on metabolic and functional changes in the brain during and after cardiac arrest. A model of asphyxial cardiac arrest in rats developed by the applicant and his sponsor will be used. Modern NMR methods will be applied for noninvasive in vivo evaluation of parameters of energy metabolism, cerebral blood flow, and ion/water disbalance. Interleaved 31P/1H NMR spectroscopy will be used to estimate ATP, phosphocreatine, inorganic phosphate, lactate, N-acetyl-L-aspartate content, and intracellular pH in the brain. Swelling pattern and distribution and intensity of cerebral blood flow will be measured by precise ADC and perfusion mapping of the brain based on contemporal newly-developed imaging techniques. Animals' survival, histological and neurological outcomes will also be evaluated. The optimal combination of inhibitors of lipid peroxidation with various mechanisms of action for effective treatment of global ischemia during cardiac arrest will be determined as a result of proposed study.

在美国，大约500,000次心脏死亡已注册 每年。 全球脑缺血是主要的病理生理事件 这将可用于救援的时间限制为5-10分钟。 这 研究研究了抗氧化疗法对代谢和 心脏骤停期间和之后大脑的功能变化。 一个 申请人开发的大鼠窒息心脏骤停模型和 他的赞助商将被使用。 现代NMR方法将用于 能量代谢参数的无创体内评估， 脑血流和离子/水破坏。交织的31p/1h nmr 光谱法将用于估计ATP，磷酸蛋白，无机 磷酸盐，乳酸，N-乙酰基-L-天冬氨酸含量和细胞内pH 在大脑中。 肿胀模式和分布和强度 脑血流将通过精确的ADC和灌注来测量 基于当代新开发的成像的大脑映射 技术。 动物的生存，组织学和神经学结果 也将评估。脂质抑制剂的最佳组合 用各种有效治疗的作用机理过氧化 结果将确定心脏骤停期间的全球性缺血 拟议的研究。