PROTEIN/DNA INTERACTIONS IN THE YEAST GENOME

酵母基因组中蛋白质/DNA 相互作用

• 批准号: 2701861
• 负责人: ROBERT T SIMPSON
• 金额: $ 13.64万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2701861
• 关键词: DNA; DNA binding protein; Saccharomyces cerevisiae; chromatin; fungal genetics; intermolecular interaction; methyltransferase

DESCRIPTION: (Applicant's abstract) The yeast genome sequence is now known, a major technical accomplishment that offer unprecedented opportunities for study of the cell biology of the simplest eukaryote. Knowledge of the interactions of regulatory and structural proteins with DNA in vivo is important for application-of the yeast DNA sequence to its function. This proposal addresses how to accomplish that goal. It builds on emerging applications of DNA methyltransferases to map protein-DNA interactions in living cells by developing methodology that will allow resolution (<l0 base pairs) study of interactions over extended regions of the S. cerevisiae genome. It describes methodology which will allow quantitative assessment of protein-DNA interactions with automated techniques that are appropriate for large regions of the yeast genome and exportable to larger eukaryotes. To illustrate the utility of the approach, we will map the chromatin organization of about half of chromosome III. Comparing portions of the structure with that determined by classical methods. Many other laboratories are highly interested both in chromatin structure and its role in DNA function and in interactions of regulatory proteins with DNA. To facilitate the research of these scientists, we propose a service which will provide chromatin mapping data for other regions of the yeast genome of interest.

描述：（申请人的摘要）酵母基因组序列现在已知， 一个主要的技术成就，为前所未有的机会 最简单的真核生物的细胞生物学研究。 了解 调节和结构蛋白与体内DNA的相互作用 对于酵母DNA序列的应用至关重要。 这 提案解决了如何实现这一目标。 它建立在新兴 DNA甲基转移酶在映射蛋白-DNA相互作用中的应用 通过开发将允许分辨率的方法学细胞（<l0碱基 成对）研究酿酒酵母扩展区域的相互作用 基因组。 它描述了将允许定量评估的方法论 与合适的自动化技术的蛋白质-DNA相互作用 对于酵母基因组的大区域，可导出到较大的真核生物。 为了说明方法的实用性，我们将绘制染色质 大约一半的III染色体的组织。 比较部分 结构由经​​典方法确定。 许多其他 实验室对染色质结构及其作用都非常感兴趣 在DNA功能以及调节蛋白与DNA的相互作用中。 到 促进这些科学家的研究，我们提出一项服务，将 为其他酵母基因组的其他区域提供染色质映射数据 兴趣。