STRUCTURES AND FUNCTIONS OF CYS3HIS ZINC DOMAINS

CYS3HIS 锌结构域的结构和功能

• 批准号: 2464849
• 负责人: Jeremy M. Berg
• 金额: $ 21.66万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-01-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2464849
• 关键词: chemical structure; cysteine; high performance liquid chromatography; histidine; metalloproteins; molecular site; nuclear magnetic resonance spectroscopy; protein structure function; transcription factor; zinc

DESCRIPTION: (adapted from applicant's abstract) A variety of classes of proteins involved in the regulation of gene expression have been found to contain short stretches of amino acids that form small structural domains organized around bound zinc ions. Many of these proteins function via interactions with nucleic acids. Two classes that are of current interest are the CCHC-box domain proteins and the CCCH domain proteins. The CCHC-box domain was first identified as a component of proteins from retroviruses where this domain appears to play a key role in the recognition of single stranded DNA. A structural model of these interactions has been developed that will be tested via studies of fragments of a protein that binds to specific single stranded DNA sequences from trypanosomes. The CCCH domain was recognized in the protein Nup475 whose level increases in response to insulin and other growth factors. The functions of CCCH domains are not well established, but recent results suggest that they may cleave specific RNA structures. This proposal reflects a collaborative effort to characterize these two structural motifs using solution state NMR structures as well as functional and binding studies.

描述：（改编自申请人的摘要）各种类别 已经发现参与基因表达调节的蛋白质已被发现 含有短的氨基酸，形成小结构结构域 围绕结合的锌离子组织。 这些蛋白质中的许多通过 与核酸相互作用。 当前感兴趣的两个类 是CCHC-box域蛋白和CCCH结构蛋白。 CCHC-box 域首先被确定为逆转录病毒蛋白质的成分 该领域似乎在识别单个的地方起着关键作用 滞留的DNA。 这些相互作用的结构模型已经开发 这将通过研究与 来自锥虫体的特定单链DNA序列。 CCCH域 在蛋白质NUP475中被识别 胰岛素和其他生长因素。 CCCH域的功能不是 建立得很好，但最近的结果表明它们可能会裂开特定的 RNA结构。 该建议反映了协作的努力 使用溶液状态NMR结构来表征这两个结构基序 以及功能和结合研究。