ELF ELECTRIC FIELD EFFECTS ON CELL ADHESION AND GROWTH

ELF 电场对细胞粘附和生长的影响

• 批准号: 2770764
• 负责人: KENNETH J MCLEOD
• 金额: $ 26.01万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2770764
• 关键词: atomic force microscopy; cell adhesion; cell growth regulation; electric field; electromagnetic radiation; environmental radiation; extracellular matrix proteins; flow cytometry; integrins; neoplastic cell culture for noncancer research; osteocytes; osteosarcoma; radiobiology; receptor expression; tissue /cell culture

DESCRIPTION: Conflicting reports at the basic science level have polarized the scientific community on the question of whether low level, extremely low frequency electromagnetic fields are capable of significantly affecting cell or tissue behavior at intensity levels corresponding to environmental exposures. The conflicting accounts of extremely low frequency field induced effects may arise, in part, as a result of a basic assumption in most in vitro studies, which is that the principal effect of field exposure is directly at the cellular level. Recent work in our lab has demonstrated that induced, as well as fixed, electric charges at the cell-substrate interface will significantly alter the growth characteristics of anchorage dependent cells. We propose, therefore, that a principal effect of low level EMF exposure is the modification of the physico-chemical characteristics of the cell substrate by the induced electric charge, which subsequently affects the adhesion, growth characteristics and phenotypic expression of anchorage dependent cells. The objective of this four year study is to test the hypothesis that field induced charge at the cell-substrate interface is a principal mechanism by which ELF electric fields modify cell behavior, and to identify how this specific alteration of the substrate interface is transduced to the cell. Preliminary results show that ELF electric field intensities similar to those induced in the adult human by 60 Hz environmental exposures, can influence both tissue adaptation and cell activity. Such fields induce maximum surface charge densities (in vitro) of less than 2 uC/m2, yet in the absence of either a magnetic or electric field, induced positive charge densities of less than 0.6 uC/m2 will significantly affect the growth of anchorage dependent cells. Further we show that induced charge can alter extracellular matrix (ECM) protein adsorption and cell adhesion. Cellular responses to induced charge should therefore depend on the substrate and adsorbed ECM protein characteristics and on the ability of the cell to adapt its adhesion receptor distribution to accommodate the altered adhesion environment. Experiments will be performed to address each of these issues, using an in vitro system in which the intensity of both the fixed and induced electric charge can be accurately controlled. Adsorbed protein, cell adhesion, cell growth, as well as changes in integrin expression, will be assayed.

描述：基础科学层面的报道冲突已经两极分化 科学界关于低水平，极低的问题 频率电磁场能够显着影响细胞 或与环境相对应的强度水平的组织行为 暴露。 频率极低的帐户相互矛盾 诱导的影响可能部分是由于基本假设 大多数体外研究，即现场暴露的主要影响 直接在细胞水平。 我们实验室的最新工作已证明 诱导的以及固定的电荷在细胞底物处产生 界面将显着改变锚固的生长特征 依赖性细胞。 因此，我们建议，低的主要影响 电位EMF暴露是物理化学的修饰 通过诱导的电荷的细胞底物的特性，该电荷 随后影响粘附，生长特征和表型 锚定依赖细胞的表达。 这项四年研究的目的是检验领域的假设 在细胞基底界面处诱导的电荷是主要机制 哪个精灵电场会修改细胞行为，并确定如何 底物界面的特异性改变被转导到细胞。 初步结果表明，精灵电场强度类似 成年人在60 Hz环境暴露中诱导的人，可以 影响组织适应和细胞活性。 这样的领域诱导 最大表面电荷密度（体外）小于2 uc/m2，但在 没有磁场或电场，诱导正电荷 密度小于0.6 UC/m2的密度将显着影响 锚固依赖性细胞。 此外，我们表明诱导的电荷会改变 细胞外基质（ECM）蛋白吸附和细胞粘附。 细胞 因此，对诱导电荷的响应应取决于底物，并且 吸附的ECM蛋白质特征和细胞适应能力 它的粘附受体分布以适应改变的粘附 环境。 将进行实验以解决这些问题中的每一个， 使用固定和固定和 诱导的电荷可以准确控制。 吸附蛋白， 细胞粘附，细胞生长以及整联蛋白表达的变化，将 被分析。