PERTURBATION OF CELL PROCESSES BY ELF ELECTRIC FIELDS

极电场对细胞过程的扰动

基本信息

批准号：
2155162
负责人：
KENNETH J MCLEOD
金额：
$ 17.93万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-09-01 至 1996-08-31
项目状态：
已结题

项目摘要

Escalating societal concern over the risks of exposure to extremely low frequency (ELF) electromagnetic fields has been fueled by epidemiologic studies which correlate increased incidence of leukemia or birth defects with proximity to power distribution systems. Unfortunately, the phenomenologic nature of these studies make it difficult to differentiate the pathogenic potential of electromagnetic fields from interacting factors (e.g., socioeconomic) that might as well explain an accelerated rate of disease. Conflicting reports at the basic science level have polarized the scientific community on this issue, resulting in only a weak acknowledgement of any potential hazards of 60 Hz electromagnetic exposure. Fundamentally, it is not yet clear if ELF, low intensity field exposure can modulate cell behavior, and if so whether specific characteristics of the field (frequency, intensity, duration) will differentially affect the response. Further, it is not known if a cell's interaction with the field is selective; whether fields perturb the activity of some cell types while remaining innocuous to others. To establish the extent to which ELF electromagnetic fields can influence cell behavior, it is essential to conduct a systematic, controlled study in which well defined (in both biological and physical terms) cell types are exposed to uniform fields of known frequency, intensity and duration, and the subsequent cellular response quantified. The objective of this four year study is to isolate those specific aspects of ELF electric fields (frequency, intensity, duration) which modulate cell behavior, and to identify the biophysical and morphologic properties of a cell which will promote its susceptibility to this exposure. Our preliminary results, at both the in vivo and in vitro level, demonstrate that extremely low intensity (less than 100 mu V/cm), frequency specific (10-100 Hz) electric fields can be a potent influence on both tissue adaptation and cell activity. Importantly, these results also suggest that the field:cell interaction depends not only on the characteristics of the field, but also on the size, shape, and surface characteristics of the cell which is being exposed. Ironically, it may well be this morphologic specificity which is responsible for the inconsistent and disparate reports of electric field interactions with biologic systems. We propose that the capacity of an electric field to modulate cell behavior is dependent not only on the frequency and strength of the field, but that the interaction is strongly dependent on the physical properties of the cell which is exposed. These experiments will be performed in an in vitro system in which the intensity of the induced electric field is both uniform and carefully controlled. Representative cell types of specific size, shape and surface properties will be examined. Biochemical and morphologic assays will be used to quantify electric field interactions.

对暴露于极低的风险的社会关注不断提高频率（ELF）电磁场已经通过流行病学促进将白血病或先天缺陷的发病率增加的研究靠近电源分配系统。不幸的是，这些研究的现象学性质使得很难区分电磁场相互作用的致病潜力可能也可以解释加速的因素（例如社会经济）疾病率。基础科学层面的报道相互矛盾在这个问题上两极化科学界，仅导致一个弱确认60 Hz电磁的任何潜在危害接触。从根本上说，尚不清楚小精灵，低强度场是否暴露可以调节细胞行为，如果是的，是否具体该场的特征（频率，强度，持续时间）将差异影响响应。此外，尚不清楚是否有单元格与该场的相互作用是选择性的；字段是否扰动某些细胞类型的活性，而其他细胞类型则无害。到确定精灵电磁场可以影响多大的程度细胞行为，必须进行系统，对照研究其中定义得很好（以生物学和物理术语）类型暴露于已知频率，强度和持续时间的均匀场，随后的细胞反应定量。这四年研究的目的是隔离这些特定方面调节的精灵电场（频率，强度，持续时间）细胞行为，并识别生物物理和形态学特性一个细胞，该单元将促进其对这种暴露的敏感性。我们的在体内和体外水平上，初步结果表明强度极低（小于100 mu v/cm），频率比（10-100 Hz）电场可能对两组的有效影响适应和细胞活性。重要的是，这些结果也表明领域：细胞相互作用不仅取决于田地，但也取决于大小，形状和表面特征正在暴露的细胞。具有讽刺意味的是，很可能是这种形态学造成不一致和不同的特异性电场与生物系统相互作用的报告。我们建议电场调节细胞行为的能力是不仅取决于场地的频率和强度，还取决于相互作用很大程度上取决于暴露的细胞。这些实验将在体外进行诱导电场强度的系统均为统一和仔细控制。特定的代表性细胞类型将检查尺寸，形状和表面特性。生化和形态测定将用于量化电场相互作用。