NOVEL PEPTIDE PRODUCT OF THE GHRH

GHRH 的新型肽产品

• 批准号: 2668606
• 负责人: Ora H Pescovitz
• 金额: $ 22.62万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2668606
• 关键词: Sertoli cells; growth hormone releasing hormone; hormone receptor; immunocytochemistry; molecular cloning; peptide hormone biosynthesis; peptide structure; protein structure function; radioimmunoassay; spermatogenesis; western blottings

DESCRIPTION (Adapted from the Investigator's abstract): Growth hormone releasing hormone (GHRH) is a member of a family of related gut, brain and gonadal peptides that includes PACAP and VIP. All the genes in this family produce two or more biologically active peptide products except for GHRH, which is known to make only one functionally significant peptide. The applicant recently discovered that the GHRH gene actually produces two distinct peptides: GHRH and a novel C-terminal peptide, the applicant has called GHRH-related peptide (GHRH-RP). GHRH-RP immunoreactivity was found to be abundant in hypothalamus and testes, two tissues which also produce GHRH. In testes GHRH-RP acts to stimulate the expression of stem cell factor (SCF), a sertoli cell products required for normal spermatogenesis. This proposal is deigned to initiate characterization of this newly described peptide product. The applicant proposes to determine the general tissue distribution of GHRH-RP by immunoassay, immunohistochemistry, and Western blot analysis. The binding characteristics of GHRH-RP to Sertoli cells will be studied by radioreceptor assay and in vitro autoradiography. The intracellular signaling pathways activated by GHRH-RP will be evaluated and using molecular cloning techniques the GHRH-RP receptor will be cloned. Finally the role of GHRH-RP in the paracrine regulation of spermatogenesis will be determined.

描述（改编自调查员的摘要）：生长激素 释放激素（GHRH）是相关肠道，大脑和 包括PACAP和VIP在内的性腺肽。 这个家庭中的所有基因 除GHRH外，生产两种或多种具有生物活性的肽产品 已知只能使一个具有功能意义的肽。 这 申请人最近发现GHRH基因实际上产生了两个 不同的肽：GHRH和一种新型的C末端肽，申请人具有 称为GHRH相关的肽（GHRH-RP）。 发现GHRH-RP免疫反应性 在下丘脑和睾丸中富含 GHRH。 在睾丸中，GHRH-RP起作用以刺激干细胞的表达 因子（SCF），一种正常精子发生所需的Sertoli细胞产品。 该提议被否决以启动该新的特征 描述了肽产品。 申请人建议确定一般 通过免疫测定，免疫组织化学和GHRH-RP的组织分布 Western印迹分析。 GHRH-RP与Sertoli的结合特征 细胞将通过辐射受体测定和体外放射自显影研究。 将评估由GHRH-RP激活的细胞内信号通路 并使用分子克隆技术将GHRH-RP受体克隆。 最后，GHRH-RP在精子发生的旁分泌调节中的作用 将确定。