RELAXIN AS A PARACRINE HORMONE

放松作为一种旁分泌激素

• 批准号: 6240074
• 负责人: Gillian Doreen Bryant-Greenwood
• 金额: $ 1.52万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-04-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6240074
• 关键词: RNase protection assay; autocrine; autoradiography; birth; decidua; embryo /fetus membrane; gene expression; genetic translation; hormone receptor; hormone regulation /control mechanism; human tissue; immunocytochemistry; in situ hybridization; membrane model; messenger RNA; paracrine; placenta; pregnancy; relaxin

The potential of the human decidua/fetal membrane system as an accessible autocrine/paracrine model will be further explored, in order to elucidate the role of relaxins in the local control of collagenolysis and the rupture of the fetal membranes at parturition. An added challenge is our finding that the second relaxin gene (H1) is expressed in this system. This will be accomplished by studying the four components of the relaxin system: gene expression, control of secretion, biological effects and receptor distribution. * Relaxin H1 and H2 gene expression an the translated products, will be identified in the endometrium/decidua, fetal membranes and placenta with specific riboprobes and antibodies throughout the cycle and pregnancy. * The effect on decidual cell relaxin secretion and the mRNA levels for relaxins by a range of decidual and placental hormones will be studied in vitro. * The biological effects of human relaxin H2 on fetal membrane/decidual production of key collagenolytic enzymes and inhibitors will be sought both in vitro and in vivo. * Changes in the concentration of relaxin receptors in intrauterine tissues will be studied through the cycle and gestation by quantitative autoradiography and the results related to those from the experiments above. The combined results will provide insights into significant problems, the local control of the accommodation of the fetal membranes to the growing fetus in the last weeks of pregnancy, and the timing of normal spontaneous membrane rupture. These studies will lead to a greater understanding of premature rupture of the fetal membranes, a major cause of premature birth which results in infants with a high incidence of neurodevelopmental impairment.

人类蜕膜/胎膜系统作为可利用的系统的潜力 将进一步探索自分泌/旁分泌模型，以阐明 松弛素在局部控制胶原溶解中的作用和 分娩时胎膜破裂。 另一个挑战是我们 发现第二个松弛素基因 (H1) 在该系统中表达。 这将通过研究松弛素的四个成分来实现 系统：基因表达、分泌控制、生物效应和 受体分布。 * 松弛素 H1 和 H2 基因表达和翻译产物将 在子宫内膜/蜕膜、胎膜和胎盘中发现 整个周期和怀孕期间的特异性核糖核酸探针和抗体。 * 对蜕膜细胞松弛素分泌和 mRNA 水平的影响 将研究一系列蜕膜激素和胎盘激素产生的松弛素 体外。 * 人松弛素H2对胎膜/蜕膜的生物学效应 将寻求生产关键的胶原蛋白分解酶和抑制剂 体外和体内。 * 宫内松弛素受体浓度的变化 将通过定量研究整个周期和妊娠期的组织 放射自显影和与实验相关的结果 多于。 综合结果将提供对重大问题的见解， 局部控制胎膜对生长的调节 胎儿在​​怀孕最后几周的情况以及正常的时间 自发膜破裂。 这些研究将带来更大的 了解胎膜早破的主要原因 早产导致婴儿的高发病率 神经发育障碍。