RELAXIN AS A PARACRINE HORMONE

放松作为一种旁分泌激素

• 批准号: 3734326
• 负责人: GILLIAN D BRYANT-GREENWOOD
• 金额: --
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734326
• 关键词: RNase protection assay; autocrine; autoradiography; birth; decidua; embryo /fetus membrane; gene expression; genetic translation; hormone receptor; hormone regulation /control mechanism; human tissue; immunocytochemistry; in situ hybridization; membrane model; messenger RNA; paracrine; placenta; pregnancy; relaxin; RNase protection assay; autocrine; autoradiography; birth; decidua; embryo /fetus membrane; gene expression; genetic translation; hormone receptor; hormone regulation /control mechanism; human tissue; immunocytochemistry; in situ hybridization; membrane model; messenger RNA; paracrine; placenta; pregnancy; relaxin

The potential of the human decidua/fetal membrane system as an accessible autocrine/paracrine model will be further explored, in order to elucidate the role of relaxins in the local control of collagenolysis and the rupture of the fetal membranes at parturition. An added challenge is our finding that the second relaxin gene (H1) is expressed in this system. This will be accomplished by studying the four components of the relaxin system: gene expression, control of secretion, biological effects and receptor distribution. * Relaxin H1 and H2 gene expression an the translated products, will be identified in the endometrium/decidua, fetal membranes and placenta with specific riboprobes and antibodies throughout the cycle and pregnancy. * The effect on decidual cell relaxin secretion and the mRNA levels for relaxins by a range of decidual and placental hormones will be studied in vitro. * The biological effects of human relaxin H2 on fetal membrane/decidual production of key collagenolytic enzymes and inhibitors will be sought both in vitro and in vivo. * Changes in the concentration of relaxin receptors in intrauterine tissues will be studied through the cycle and gestation by quantitative autoradiography and the results related to those from the experiments above. The combined results will provide insights into significant problems, the local control of the accommodation of the fetal membranes to the growing fetus in the last weeks of pregnancy, and the timing of normal spontaneous membrane rupture. These studies will lead to a greater understanding of premature rupture of the fetal membranes, a major cause of premature birth which results in infants with a high incidence of neurodevelopmental impairment.

人类Decidua/胎儿膜系统的潜力作为可访问的 将进一步探索自分泌/旁分泌模型，以阐明 松弛素在胶原完全溶解和局部控制中的作用 分娩时胎儿膜破裂。 一个额外的挑战是我们的 发现第二种放松基因（H1）在该系统中表达。 这将通过研究松弛素的四个组成部分来完成 系统：基因表达，分泌的控制，生物学作用和 受体分布。 *放松蛋白H1和H2基因表达，翻译的产品将是 在子宫内膜/deciDUA，胎儿膜和胎盘中鉴定 在整个周期和怀孕期间，具体的核糖核酸和抗体。 *对decIDual细胞松弛素分泌的影响和mRNA水平的影响 将研究一系列判决和胎盘激素的松弛素 体外。 *人松弛素H2对胎膜/deciDual的生物学作用 将寻求关键胶原式酶和抑制剂的产生 体外和体内。 *宫内松弛素受体浓度的变化 将通过定量研究组织和妊娠。 放射自显影及与实验的结果有关 多于。 结合结果将为重大问题提供见解， 局部控制胎儿膜到生长 胎儿在​​怀孕的最后几周和正常的时机 自发的膜破裂。 这些研究将导致更大的 了解胎儿过早破裂，这是主要原因 早产，导致婴儿发病率高 神经发育障碍。