ORGANOTINS AS POSSIBLE LARVICIDES/INSECTICIDES TO CONTROL MALARIA

有机锡作为可能的杀幼虫剂/杀虫剂来控制疟疾

基本信息

批准号：
6239892
负责人：
GEORGE ENG
金额：
$ 16.13万
依托单位：
UNIVERSITY OF THE DISTRICT OF COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-01 至 1998-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6239892
关键词：
Culicidae Mossbauer spectrometry Plasmodium berghei chemical structure function chemical synthesis communicable disease control infrared spectrometry insecticides laboratory mouse larva malaria microinjections organic chemicals tin toxicant screening

项目摘要

The primary objective of the research is to investigate the use of organotin compounds as potential insecticides and/or larvicides against the Anopheles mosquito, the primary vector of the parasite responsible for human malaria. The development of an effective insecticide and/or larvicide would be of tremendous value in the effort to reduce the spread of this highly debilitating and often deadly disease. To accomplish this objective, organotin compounds will be tested for their effectiveness against mosquito larvae, adult female mosquitoes and mosquitoes infected with Plasmodium berghei, a rodent specific malaria. Larvicidal screening will involve placing mosquito larvae and various concentrations of the organotins together for 24 hours and determining the toxic effects of the organotin compounds. Three methods will be used to determine the effects of the organotin compounds on adult female mosquitoes. The first method involves a topical application of the compounds to the thorax of the anesthetized mosquito and determining the effects. The second procedure involves placing adult mosquitoes into cartons that have been treated with the organotin compound of interest for 24 hours. Finally, the effects of the compounds on the malaria protozoan parasite development will be evaluated by following the development of the oocyst and sporozoite stages of Plasmodium berghei, a rodent specific malaria. Subsequently, new organotin compounds will be designed and synthesized with the aim of developing more effective compounds. The proposed research is also designed to provide training for students at the University of the District of Columbia in the research methods currently employed at government, industrial and university laboratories. To accomplish this, students will participate in all phases of the project, from the syntheses of organotin compounds to the toxicity studies involving both mosquito larvae and adult mosquito.

该研究的主要目的是调查使用有机锡化合物作为潜在的杀虫剂和/或杀幼虫剂按蚊，寄生虫的主要媒介人类疟疾。开发有效的杀虫剂和/或杀幼虫剂对于减少传播具有巨大价值这种使人高度衰弱且往往致命的疾病。为了实现这一点客观地，将测试有机锡化合物的有效性预防蚊子幼虫、成年雌蚊和蚊子感染伯氏疟原虫，一种啮齿类动物特有的疟疾。杀幼虫筛选将涉及放置蚊子幼虫和不同浓度的将有机锡放在一起 24 小时并确定其毒性作用有机锡化合物。将使用三种方法来确定效果有机锡化合物对成年雌性蚊子的影响。第一种方法涉及将化合物局部应用于胸部麻醉蚊子并确定效果。第二道程序包括将成年蚊子放入经过处理的纸箱中感兴趣的有机锡化合物24小时。最后，效果如下对疟疾原生动物寄生虫发育的化合物将是通过跟踪卵囊和子孢子阶段的发育进行评估伯氏疟原虫，一种啮齿类动物特有的疟疾。随后，新设计和合成有机锡化合物的目的是开发更有效的化合物。拟议的研究还旨在为学生提供培训哥伦比亚特区大学的研究方法目前受雇于政府、工业和大学实验室。为了实现这一目标，学生将参与课程的所有阶段项目，从有机锡化合物的合成到毒性研究包括蚊子幼虫和成蚊。