GENETIC ANALYSIS OF LEARNING PERFORMANCE

学习表现的遗传分析

基本信息

批准号：
2415291
负责人：
BRIAN H. SMITH
金额：
$ 8.39万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1995
资助国家：
美国
起止时间：
1995-05-01 至 1999-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2415291
关键词：
Insecta association learning behavioral /social science research tag behavioral genetics behavioral habituation /sensitization discrimination learning genetic markers linkage mapping male molecular genetics octopamine olfactions operant conditionings performance recombinant DNA reversal learning serotonin

项目摘要

Studies outlined below will investigate the quantitative and molecular genetic bases for variation in learning performance in the honey bee, Apis mellifera. Research into the bases of learning and memory in invertebrates such as mollusks and insects has demonstrated that fundamental properties of behavioral plasticity developed in studies of vertebrates apply across a broad phylogenetic spectrum. Yet, the genetic bases for learning traits are only beginning to be studied. Honey bees provide excellent opportunities for studying genetic factors that influence learning behavior because of the potential to apply several powerful behavioral paradigms for studying learning in vertebrates. The series of proposed studies will take advantage of the ability to train and analyze honey bee drones (males). Drones arise from unfertilized eggs and are thus haploid recombinants of the maternal genotype. Quantitative genetic selection on a haploid proceeds faster than it would on a diploid. Furthermore, linkage mapping from haploid genotypes using a map recently developed from a series of RAPD primers will not have the complications of such mapping using diploid genotypes. The specific aims of the studies are: * To further determine the quantitative genetic bases underlying variability in olfactory learning performance. Lines will be selected for fast versus slow reversal learning performance relative to unselected control lines. Use of this conditioning protocol and inclusion of appropriate control procedures will allow us to test whether associative learning performance can be selected without correlated responses in sensitization or motor systems. * To investigate the molecular genetic background using a linkage map established from RAPD genetic markers. These kinds of analyses will be carried out in recombinant F2 drone progeny of hybrid queens, in which spurious correlations due to founder effects in small base populations would be attenuated.

下面概述的研究将研究定量和分子蜂蜜蜜蜂学习表现差异的遗传基础，API Mellifera。研究无脊椎动物的学习和记忆基础例如软体动物和昆虫已经证明了基本特性在脊椎动物研究中发展的行为可塑性适用于广泛的系统发育谱。但是，学习特质的遗传基础才开始研究。蜂蜜蜜蜂提供了极好的研究影响学习的遗传因素的机会由于有可能采用多种强大行为的行为用于研究脊椎动物学习的范例。一系列提议研究将利用训练和分析蜜蜂的能力无人机（男性）。无人机由未施用的卵产生，因此是单倍体母体基因型的重组。定量遗传选择单倍体进行的速度比二倍体上的速度快。此外，链接使用最近从A 一系列RAPD底漆将没有这种映射的并发症使用二倍体基因型。研究的具体目的是： *进一步确定基础的定量遗传基础嗅觉学习绩效的可变性。将选择行相对于未选择的快速与缓慢的逆转学习表现控制线。使用此条件方案和包含适当的控制程序将使我们能够测试是否关联可以在没有相关响应的情况下选择学习表现灵敏度或电机系统。 *使用连锁图研究分子遗传背景由RAPD遗传标记建立。这些分析将是在重组F2无人机后代的混合皇后时代进行，其中由于基本小种群的创始人效应而引起的虚假相关性会减弱。