SERODIAGNOSIS AND IMMUNOPROPHYLAXIS OF AMEBIASIS

阿米巴病的血清学诊断和免疫预防

• 批准号: 2538212
• 负责人: David M. Lyerly
• 金额: $ 33.85万
• 依托单位: TECHLAB, INC.
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 2000-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2538212
• 关键词: SERODIAGNOSIS; IMMUNOPROPHYLAXIS; AMEBIASIS

DESCRIPTION (Adapted from the Applicant's Abstract): The trophozoites of Entamoeba histolytica, the cause of human amebiasis adhere to colonic mucins, intestinal epithelium and leukocytes via a galactose-specific lectin. The lectin is a major conserved surface antigen which is recognized by the immune sera of 99 percent of tested patients with amebic liver abscess and colitis. Inhibition of this lectin with galactose prevents adherence and contact- dependent killing of immune effector cells such as neutrophils and macrophages. The lectin also mediates amebic resistance to the human complement membrane attack complex. Preliminary evidence indicate that the recombinant-expressed lectin is a more specific serodiagnostic reagent for acute amebiasis. In addition, the recombinant lectin is a protective antigen in an animal model of amebiasis. Dr. Lyerly proposes the development a more specific EIA for serodiagnosis of amebiasis using recombinant expressed amebic adherence lectin. Experience acquired in expression of fragments of the lectin gene for the EIA will be applied towards future development of a vaccine against amebiasis..

描述（改编自申请人的摘要）：滋养体 人类amebiasis的原因持续到结肠 通过半乳糖特异性的粘蛋白，肠上皮和白细胞 凝集素。凝集素是一种主要保守的表面抗原 由99％的测试患者的免疫血清识别 阿米比克肝脓肿和结肠炎。抑制该凝集素 半乳糖防止免疫的依从性和接触依赖性杀害 效应细胞，例如中性粒细胞和巨噬细胞。凝集素也是如此 介导对人类补体膜攻击 复杂的。初步证据表明重组表达 凝集素是用于急性amebiasis的更特异性的血清诊断试剂。 另外，重组凝集素是动物中的保护性抗原 阿米巴病模型。 Lyerly博士提出了该开发的更具体的EIA，用于塞毒素诊断 使用重组表达的Amebic依从性凝集素的Amebiasis。 在表达凝集素基因片段表达的经验 EIA将用于未来开发针对的疫苗 amebiasis ..