AUTOMATED MICROCHIP DNA ANALYSIS SYSTEM

自动化微芯片 DNA 分析系统

• 批准号: 2840529
• 负责人: STEVAN B JOVANOVICH
• 金额: $ 13.6万
• 依托单位: MOLECULAR DYNAMICS, INC.
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2840529
• 关键词: DNA; bacteriophage M13; biomedical automation; biomedical equipment development; computer program /software; genetic polymorphism; genome; genotype; nucleic acid sequence; plasmids; polymerase chain reaction

This project will develop an Automated DNA Analysis Microchip System that will load, run, and detect DNA samples in microchannels on microchips. The system will be applied to analyze single nucleotide polymorphisms (SNPs), genotype fragments, and read DNA sequences, all at very high throughput rates. The system will consist of microchips and a robotic instrument currently under development. In a typical application, the instrument will transfer samples from microtiter plates to sample loading ports on the microchips. The loaded microchips will be moved to dock with electrodes under a detector. The samples will them be injected into separation microchannels and electrophoresed past a detector where the fluorescence is measured. The microchips will contain microchannels for both sample injection and separation. In Phase I, we plan to characterize the existing prototype and extend its functionality. We will design and build microchips with up to 48 channels for DNA sequencing. The system will be tested with M13 controls and with panels of test real-world sequencing samples, including M13 clones, plasmids, and PCR fragments. PROPOSED COMMERCIAL APPLICATION: The project will develop a very high throughput DNA analysis system for DNA sequencing and genotyping using a microchip-based format. This system will have widespread applications in biomedical research,, basic research and in applied areas such as DNA diagnostics, forensics, animal and plant typing, and human identification. The customers will be genome centers, pharmaceutical and biotechnology companies, universities, and diagnostic laboratories.

该项目将开发自动 DNA 分析微芯片系统 它将加载、运行和检测微通道中的 DNA 样本 微芯片。 该系统将用于分析单核苷酸 多态性 (SNP)、基因型片段和读取 DNA 序列，全部 以非常高的吞吐率。该系统将由微芯片和 目前正在开发的机器人仪器。 在一个典型的 应用程序中，仪器将从微量滴定板转移样品 到微芯片上的样品加载端口。 加载的微芯片将 被移动到与探测器下方的电极对接。 样品将 将它们注入分离微通道并电泳过去 测量荧光的检测器。 微芯片将 包含用于样品注射和分离的微通道。同相 I，我们计划表征现有原型并扩展其 功能。 我们将设计和制造多达 48 个微芯片 DNA 测序通道。 该系统将使用M13进行测试 控制和测试真实世界测序样本的面板， 包括 M13 克隆、质粒和 PCR 片段。 拟议的商业应用： 该项目将开发一种非常高通量的 DNA 分析系统 使用基于微芯片的格式进行 DNA 测序和基因分型。 这 系统将在生物医学研究、基础研究等方面有广泛的应用 DNA 诊断、法医学、动物学等研究和应用领域 以及植物分型和人类鉴定。 客户将是 基因组中心、制药和生物技术公司、 大学和诊断实验室。