SYNAPTIC VESICLE FUSION, RECYCLING & SYNAPTIC REMODELING

突触小泡融合、回收

• 批准号: 2676686
• 负责人: MARK S PERIN
• 金额: $ 22.2万
• 依托单位: CLEVELAND CLINIC FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2676686
• 关键词: Drosophilidae; chemical binding; developmental neurobiology; endocytosis; enzyme activity; immunoelectron microscopy; immunofluorescence technique; intracellular transport; laboratory rat; membrane fusion; molecular cloning; neural plasticity; phospholipase A2; protein isoforms; protein transport; receptor; receptor binding; recombinant proteins; reptile poison; synaptic vesicles; synaptogenesis; synaptotagmin

DESCRIPTION: (adapted from Applicant's Abstract) The aim of this application is the elucidation of the roles of potential key elements in mechanisms of synaptic vesicle fusion, recycling and synaptic remodeling. This will be accomplished with two sets of studies connected by their involvement in the membrane traffic of synaptic terminals. The first set of studies focuses on the interaction between the synaptic vesicle protein, synaptotagmin, and the presynaptic proteins, the neurexins, as a mechanism for docking and fusion. The second set of studies focuses on two novel proteins of 47 and 49 kD that bind a snake venom toxin, taipoxin, that blocks synaptic vesicle recycling. The investigators hypothesize that these two proteins are directly involved with uptake of synaptic material that may be part of a mechanism for synaptic remodeling.

描述：（改编自申请人的摘要） 应用是阐明潜在关键元素的作用 突触囊泡融合，回收和突触的机制 重塑。 这将通过两组研究来完成 通过参与突触的膜流量连接 终端。 第一组研究的重点是 突触囊泡蛋白，突触毒素和突触前 蛋白质，神经毒素，作为对接和融合的一种机制。 这 第二组研究的重点是两个47和49 kD的新型蛋白质 结合蛇毒毒素taipoxin，阻塞突触囊泡 回收。 研究人员假设这两种蛋白质是 直接参与可能是一部分的突触材料的吸收 突触重塑的机制。

项目成果

The COOH terminus of synaptotagmin mediates interaction with the neurexins.

• DOI: 10.1016/s0021-9258(17)37233-2
• 发表时间: 1994-03
• 期刊: The Journal of biological chemistry
• 作者: M. Perin

Mirror image motifs mediate the interaction of the COOH terminus of multiple synaptotagmins with the neurexins and calmodulin.

镜像基序介导多个突触结合蛋白的COOH末端与神经毒素和钙调蛋白的相互作用。

• DOI: 10.1021/bi960853x
• 发表时间: 1996
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Perin,MS