CORE--MOLECULAR ANALYSIS

核心--分子分析

• 批准号: 6234500
• 负责人: Sue Tilton Griffin
• 金额: $ 12.16万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6234500
• 关键词: amyloid proteins; analytical method; animal genetic material tag; animal tissue; apolipoproteins; biomedical facility; enzyme linked immunosorbent assay; human genetic material tag; human tissue; in situ hybridization; interleukin 1; messenger RNA; molecular biology; neurotrophic factors; northern blottings; tissue /cell culture; western blottings

The purpose of Core C is to provide molecular analysis services to the Project investigators in this Program Project. Western immunoblot, northern and in situ hybridization analyses (to determine the levels of IL-1, S100beta, and beta-APP and their encoding mRNAs, as well as other proteins that may be of interest, e.g., TGF-beta, and FGF) will be available upon request; S100beta and GFAP ELISAs will also be available for rapid screening of large numbers of samples. The levels of total protein, total RNA, and ribosomal RNA will be performed as routine analyses necessary for determining the relative levels of the specific proteins and mRNAs mentioned. The human tissue to be analyzed for studies in Project 1 will have been obtained by Project investigators from the Neuropathology Core (B1 and B2). The animal tissue or cells will be from rats used in experiments in Projects 2 and 3. Data from analyses performed in this Core will be provided to the Project Investigators as both raw data and preliminarily analyzed data. For example, data from northern and western immunoblot analyses will be returned to the investigator as raw data (pictures of gels and films of transfers) and ng/micrograms tissue or ng/micrograms of extract. Data from ELISAs will be returned as raw data in the form of optical density units per sample, together with a standard curve generated at the same time, and calculation of ng/micrograms protein and ng/micrograms extract. Following in situ hybridization, sections will be returned to the investigator or sent to Core D for analysis. Data from in situ hybridization of cultures will be returned to the investigator as cpm/well. Values from studies of human and animal tissue will be entered into the relevant database.

核心C的目的是为 该计划项目中的项目调查人员。西方免疫印迹， 北部和原位杂交分析（确定 IL-1，S100BETA和BETA-APP及其编码mRNA以及其他 可能感兴趣的蛋白质，例如TGF-BETA和FGF） 可应要求提供； S100beta和GFAP ELISA也将提供 用于快速筛选大量样品。总数 蛋白质，总RNA和核糖体RNA将作为常规进行 确定特定的相对水平所需的分析 提到蛋白质和mRNA。进行研究的人体组织 项目1将由项目调查人员获得 神经病理学核心（B1和B2）。动物组织或细胞将来自 项目2和3的实验中使用的大鼠。分析的数据 在此核心中执行的项目调查人员将提供 原始数据和初步分析数据。例如，来自 北部和西部免疫印迹分析将返回到 研究者作为原始数据（凝胶和转移胶片的图片）和 Ng/micrograms组织或NG/微克的 提炼。来自ELISA的数据将以原始数据的形式返回 每个样品的光密度单元，以及标准曲线 同时生成，并计算Ng/micrograms蛋白质和 NG/微克提取物。遵循原位杂交，部分将是 返回调查员或发送到核心D进行分析。来自 培养物的原位杂交将退还给研究者 作为CPM/井。人类和动物组织的研究值将是 输入相关数据库。