ELECTRON TRANSFER MECHANISMS IN REACTION CENTERS

反应中心的电子传输机制

• 批准号: 2331972
• 负责人: PETER LESLIE DUTTON
• 金额: $ 21.42万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2331972
• 关键词: Rhodopseudomonas; X ray crystallography; bacterial proteins; bioenergetics; chlorophyll; coulometry; crystallization; cytochrome c; cytochrome oxidase; electric field; electrochemistry; electron transport; flash photolysis; heme; hydropathy; molecular energy level; molecular film; photosynthetic bacteria; photosynthetic reaction centers; protein engineering; protein structure function; site directed mutagenesis; spectrometry; synthetic peptide; thermodynamics

It is now well known that the central function of the bioenergetic machinery of respiratory and photosynthetic membranes is to convert the free energy between oxidants and reductants into a transmembrane electrical potential and a proton gradient, and that this drives essential energy requiring processes of ion and metaholite transport and ATP production. A challenge is to understand how electron transfers are controlled in these membrane proteins that are crowded with highly reactive intermediates. The aim of the proposed experimental work is to determine the fundamental principles of molecular design and engineering that are responsible for the governance of the rates and directional specificity of electron transfers through protein directed across bioenergetic membranes. The Langmuir-Blodgett film balance and self-assembled monolayers will be used to manipulate the proteins into a vectorial configuration that facilitates structure determination and ready investigation of charge separating events. These assemblies will be used to monitor, modulate and activate charge separating events in respiratory and photosynthetic redox proteins. A second approach will develop novel synthetic constructions of protein and cofactors, designed and engineered to provide minimal water soluble structures that perform critical functions of the native protein. It is hoped in the long term that these synthetic redox proteins will access problems difficult, if not impossible, with the membrane proteins, including offering a simpler path towards crystallization and structural resolution of the active parts of their natural counterparts. In describing the engineering parameters of electron transfer driven charge separation, the regions of normal efficient operation will be identified. Recognition of the functional tolerances, therefore, define the threshold of dysfunction that underlies pathological conditions.

众所周知，生物能的核心功能 呼吸道和光合膜的机械是转换 氧化剂和还原剂之间的自由能进入跨膜 电势和质子梯度，这驱动了必不可少的 需要离子和元荷属传输过程和ATP的能量 生产。一个挑战是了解电子传输的方式 在这些膜蛋白中控制的蛋白质高度拥挤 反应性中间体。拟议的实验工作的目的是 确定分子设计和工程的基本原理 负责利率和方向的治理 通过针对跨越蛋白质的电子转移的特异性 生物能膜。 Langmuir-Blodgett电影平衡和自组装的单层将是 用于将蛋白质操纵成矢量构型 促进结构确定和现成的指控调查 分开事件。这些组件将用于监视，调制和 激活电荷分离呼吸道和光合氧化还原中的事件 蛋白质。第二种方法将开发新的合成结构 蛋白质和辅因子，设计和设计以提供最少的水 执行天然蛋白质关键功能的可溶性结构。 长期希望这些合成的氧化还原蛋白将 膜蛋白的访问问题很难，即使不是不可能的，如果不是不可能的， 包括提供更简单的结晶和结构的路径 自然对应物的活跃部分的解决方案。 在描述电子传输驱动的工程参数时 电荷分离，正常有效操作的区域将是 确定。因此，识别功能公差定义了 基础病理状况的功能障碍的阈值。