NEUROANATOMY OF THE AUDITORY SYSTEM

听觉系统的神经解剖学

• 批准号: 2443555
• 负责人: Nell B. Cant
• 金额: $ 25.51万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2443555
• 关键词: agglutinins; auditory nuclei; auditory pathways; axon; brain cell; brain mapping; cell type; cochlea; dendrites; fluorescent dye /probe; gerbil /jird; guinea pigs; inferior colliculus; neural information processing; neuronal transport; olivary body; sectioning

Two fundamental concepts unite the proposed projects. The first is that the topographic (tonotopic) organization characteristic of auditory nuclei reflects their basic functional organization. The second is that the auditory system is made up of multiple parallel pathways arising from different neuronal types. The proposed research is designed to relate the axonal arborization patterns of specific cell types to the three- dimensional frame of reference afforded by isofrequency contours. Eleven experiments are proposed to achieve four specific aims: 1) To characterize the organization of the bushy cell pathway from the cochlear nucleus to the superior olivary complex and from the superior olivary complex to the inferior colliculus. 2) To characterize the multipolar cell path way from the ventral cochlear nucleus to the inferior colliculus. 3) To analyze the periolivary cell groups of the superior olivary complex in terms of specific cell types. 4) To examine the organization of the isofrequency contours in the inferior colliculus in terms of the arborization patterns of lemniscal inputs from the bushy and multi-polar cell pathways and from selected periolivary nuclei. The fourth specific aim provides the framework for interpretation of the results of the other studies. The hypothesis is that, although a number of lower brain stem auditory pathways converge on one isofrequency contour in the inferior colliculus, their terminal arborizations occupy different domains within that contour. Methods to be used include: l) Tracing techniques based on the anterograde and/or retrograde transport of biocytin, Phaseolus vulgaris-leucoagglutin, and a number of different fluorescent dyes, and 2) A fixed slice preparation in which the dendritic arborization patterns of neurons that project to known sources can be examined. Intrinsic plexuses of axons within the inferior colliculus appear to coincide in position and extent with the isofrequency contours defined physiologically and will serve to represent those contours anatomically. Our long-term objective is to understand the organization of the brainstem auditory pathways in terms of connections among the multiple, different neuronal types that form them. Neuroanatomical studies provide information essential for reaching an understanding of the mechanisms of auditory processing. The neuronal activity that leads to functional expression is dependent on the precise and specific connectivities among hundreds of specialized neuronal types. Detailed explications of these connectivities will not only help to explain available functional data, it will provide a basis for developing rational hypotheses about auditory function that can be tested further.

两个基本概念将拟议的项目团结起来。首先是 听觉核的地形（吨位）组织特征 反映其基本功能组织。第二个是 听觉系统由多个由多个平行途径组成 不同的神经元类型。拟议的研究旨在将 特定细胞类型的轴突树木化模式 等音轮廓提供的尺寸参考框架。十一 提出实验以实现四个具体目标：1）表征 从耳蜗核到组织的浓密细胞通路的组织 上橄榄络合物，从上橄榄络合物到 下丘。 2）表征从 腹侧耳蜗核与下丘。 3）分析 就上橄榄络合物的周围细胞组而言 特定的细胞类型。 4）检查同流的组织 根据树博模式，下丘的轮廓 来自浓密和多极性细胞通路的lemaniscal输入 选定的周围核。第四个特定目标提供了 解释其他研究结果的框架。这 假设是，尽管许多下脑干的听觉 途径在下丘中收敛于一个同源轮廓， 它们的末端植物在该轮廓内占据了不同的域。 要使用的方法包括：l）基于顺行的跟踪技术 和/或生物细胞逆运输，叶糖蛋白的叶糖蛋白阶段 和许多不同的荧光染料，以及2）固定切片 制备神经元的树突状树自动化模式 可以检查已知来源的项目。轴突的固有丛 在下丘中似乎是一致的位置和程度 通过生理定义的同源轮廓，将用于 在解剖学上表示这些轮廓。 我们的长期目标是了解脑干的组织 在多个，不同的连接方面的听觉途径 形成它们的神经元类型。神经解剖学研究提供信息 了解听觉机制的理解至关重要 加工。导致功能表达的神经元活性是 取决于数百种的精确和特定连接性 专门的神经元类型。这些连接性的详细说明 不仅会帮助解释可用的功能数据，还将提供 开发有关听觉功能的理性假设的基础 可以进一步测试。