AMINO ACID TRANSPORT AFTER SMALL BOWEL RESECTION

小肠切除术后的氨基酸转运

• 批准号: 2770453
• 负责人: HARRY CHARLES SAX
• 金额: $ 11.85万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至 2000-03-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2770453
• 关键词: alanine; aminoacid transport; antiserum; arginine; dietary aminoacid; epidermal growth factor; gastrointestinal absorption /transport; glutamine; histology; hormone regulation /control mechanism; intestinal villi; intestine surgery; jejunectomy /duodenectomy /ileectomy; laboratory rabbit; leucine; neurotensin; nutrition related tag; octreotide; postoperative state; short chain fatty acid; small intestines; somatotropin

DESCRIPTION: (Adapted from investigator's abstract) This research is designed to advance understanding of changes in active amino acid transport by the enterocyte after massive small bowel resection. The role of hormones and specific nutrients as mediators of these-changes will also be examined. The hypotheses to be tested are: 1. Active amino acid transport by the enterocyte is altered during adaptation after massive bowel resection. 2. Specific hormones influence amino acid transport during this adaptation. 3. Specific nutrients, including amino acids and short chain fatty acids (SCFA) may be beneficial after resection by improving amino acid uptake. New Zealand rabbits undergo 70% jejunoileal resection or sham operation. One week, one month, and six months later, small bowel mucosa will be processed to brush border membrane vesicles. Uptake of glutamine (GLN), arginine, alanine, and leucine will be quantified using a rapid mixing/filtration technique. Changes in plasma levels of neurotensin (NT) epidermal growth factor (EGF), and growth hormone (GH) will be determined by radioimmunoassay. Infusions of NT, EGF, GH and the somatostatin analogue octreotide will assess hormonal influence on amino acid transport. Octreotide's effect will be further verified by immuno- neutralization with specific antisera to NT, EGF, and GH. Dietary manipulation includes the provision of isonitrogenous, isocaloric diets with protein as whole protein, oligopeptides or free amino acids. SCFA will be supplemented either as free sodium butyrate or pectin. Influence of parenteral GLN on amino acid transport from both the lumen and the splanchnic circulation will also be quantified. The eventual goal is to develop nutrient/hormone combinations that will improve adaptation. It is hoped that the knowledge gained will contribute to new therapeutic strategies for patients with short gut syndrome.

描述：（改编自研究者的摘要）这项研究是 旨在促进对活性氨基酸变化的理解 大面积小肠切除术后肠上皮细胞的运输。 这 激素和特定营养素作为这些变化介质的作用 也将受到审查。 要检验的假设是： 1. 主动 肠上皮细胞的氨基酸转运在适应过程中发生改变 大肠切除术后。 2. 特定激素影响氨基 在这种适应过程中酸的运输。 3.特定营养素， 包括氨基酸和短链脂肪酸（SCFA）可能是 通过改善氨基酸的摄取对切除后有益。 新西兰兔接受了 70% 的空肠切除术或假手术。 一周、一个月、六个月后，小肠粘膜会 加工成刷状缘膜囊泡。 谷氨酰胺（GLN）的摄取， 精氨酸、丙氨酸和亮氨酸将使用快速定量 混合/过滤技术。 血浆神经降压素水平的变化 (NT) 表皮生长因子 (EGF) 和生长激素 (GH) 通过放射免疫测定法测定。 输注 NT、EGF、GH 和 生长抑素类似物奥曲肽将评估激素对氨基的影响 酸运输。奥曲肽的效果将通过免疫进一步验证 用 NT、EGF 和 GH 特异性抗血清中和。 饮食 控制措施包括提供等氮、等热量饮食 蛋白质为全蛋白、寡肽或游离氨基酸。 短链脂肪酸 将补充游离丁酸钠或果胶。影响 胃肠外 GLN 对从管腔和肠管的氨基酸转运的影响 内脏循环也将被量化。 最终目标是 开发可改善适应性的营养/激素组合。 它 希望所获得的知识将有助于新的治疗 短肠综合征患者的治疗策略。

项目成果

Octreotide diminishes luminal nutrient transport activity, which is reversed by epidermal growth factor.

奥曲肽可减少管腔营养物质转运活性，表皮生长因子可逆转这种活性。

• DOI: 10.1016/s0002-9610(96)00163-8
• 发表时间: 1996
• 期刊: American journal of surgery
• 影响因子: 3
• 作者: Seydel,AS;Miller,JH;Sarac,TP;Ryan,CK;Chey,WY;Sax,HC
• 通讯作者: Sax,HC

Glucocorticoids upregulate intestinal nutrient transport in a time-dependent and substrate-specific fashion.

• DOI: 10.1016/s1091-255x(98)80036-x
• 发表时间: 1998-09-01
• 期刊: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
• 作者: Iannoli, P;Miller, J H;Sax, H C
• 通讯作者: Sax, H C

Characterization of L-leucine transport system in brush border membranes from human and rabbit small intestine.

人和兔小肠刷状缘膜中 L-亮氨酸转运系统的表征。

• DOI: 10.1016/s0026-0495(99)90155-5
• 发表时间: 1999
• 期刊: Metabolism: clinical and experimental
• 作者: Iannoli,P;Miller,JH;Wang,HT;Bode,B;Souba,WW;Avissar,NE;Sax,HC
• 通讯作者: Sax,HC

Early nutritional support in critical illness is important.

危重疾病的早期营养支持很重要。

• DOI: 10.1016/s0749-0704(05)70269-4
• 发表时间: 1996
• 期刊: Critical care clinics
• 影响因子: 4.3
• 作者: Sax,HC

Low-dose enteral feeding is beneficial during total parenteral nutrition.

全肠外营养期间低剂量肠内喂养是有益的。

• DOI: 10.1016/s0002-9610(96)00039-6
• 发表时间: 1996
• 期刊: American journal of surgery
• 影响因子: 3
• 作者: Sax,HC;Illig,KA;Ryan,CK;Hardy,DJ
• 通讯作者: Hardy,DJ