AMINO ACID TRANSPORT AFTER SMALL BOWEL RESECTION

小肠切除术后的氨基酸转运

• 批准号: 2147976
• 负责人: HARRY CHARLES SAX
• 金额: $ 10.22万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2147976
• 关键词: alanine; aminoacid transport; antiserum; arginine; dietary aminoacid; epidermal growth factor; gastrointestinal absorption /transport; glutamine; histology; hormone regulation /control mechanism; intestinal villi; intestine surgery; jejunectomy /duodenectomy /ileectomy; laboratory rabbit; leucine; neurotensin; nutrition related tag; peptide hormone analog; postoperative state; short chain fatty acid; small intestines; somatostatin; somatotropin

DESCRIPTION: (Adapted from investigator's abstract) This research is designed to advance understanding of changes in active amino acid transport by the enterocyte after massive small bowel resection. The role of hormones and specific nutrients as mediators of these-changes will also be examined. The hypotheses to be tested are: 1. Active amino acid transport by the enterocyte is altered during adaptation after massive bowel resection. 2. Specific hormones influence amino acid transport during this adaptation. 3. Specific nutrients, including amino acids and short chain fatty acids (SCFA) may be beneficial after resection by improving amino acid uptake. New Zealand rabbits undergo 70% jejunoileal resection or sham operation. One week, one month, and six months later, small bowel mucosa will be processed to brush border membrane vesicles. Uptake of glutamine (GLN), arginine, alanine, and leucine will be quantified using a rapid mixing/filtration technique. Changes in plasma levels of neurotensin (NT) epidermal growth factor (EGF), and growth hormone (GH) will be determined by radioimmunoassay. Infusions of NT, EGF, GH and the somatostatin analogue octreotide will assess hormonal influence on amino acid transport. Octreotide's effect will be further verified by immuno- neutralization with specific antisera to NT, EGF, and GH. Dietary manipulation includes the provision of isonitrogenous, isocaloric diets with protein as whole protein, oligopeptides or free amino acids. SCFA will be supplemented either as free sodium butyrate or pectin. Influence of parenteral GLN on amino acid transport from both the lumen and the splanchnic circulation will also be quantified. The eventual goal is to develop nutrient/hormone combinations that will improve adaptation. It is hoped that the knowledge gained will contribute to new therapeutic strategies for patients with short gut syndrome.

描述：（根据调查员的摘要改编）这项研究是 旨在提高对活性氨基酸变化的了解 大肠切除后，肠肠细胞运输。 这 激素和特定营养作为这些变化的介体的作用 也将被检查。 要测试的假设是：1。 在适应过程中，通过肠肠细胞转运氨基酸转运 大肠切除后。 2。特定的激素影响氨基 在这种适应过程中，酸转运。 3。特定的营养素， 包括氨基酸和短链脂肪酸（SCFA）可能是 切除后通过改善氨基酸摄取而有益。 新西兰兔子进行70％的空肠纤维切除术或假手术。 一个星期，一个月和六个月后，小肠粘膜将是 加工以刷边界膜囊泡。 吸收谷氨酰胺（GLN）， 精氨酸，丙氨酸和亮氨酸将通过快速定量 混合/过滤技术。 血浆神经素水平的变化 （NT）表皮生长因子（EGF）和生长激素（GH）将是 由放射免疫测定法确定。 NT，EGF，GH和 生长抑素模拟奥曲肽将评估荷尔蒙对氨基的影响 酸转运。通过免疫 - 将进一步验证奥曲肽的作用 用特异性抗血清中和NT，EGF和GH。 饮食 操纵包括提供异义源，等饮食 蛋白质是全蛋白，寡肽或游离氨基酸。 SCFA 将被补充为游离丁酸钠或果胶。影响 从管腔和氨基酸转运上的肠胃外GLN 诊断循环也将被量化。 最终的目标是 开发营养/激素组合，以改善适应性。 它 希望获得的知识将有助于新的治疗 肠道综合征短的患者策略。