PATTERNING IN C ELEGANS EMBRYONIC DEVELOPMENT

线虫胚胎发育的模式

• 批准号: 2025034
• 负责人: WILLIAM B WOOD
• 金额: $ 20.9万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2025034
• 关键词: Caenorhabditis elegans; alleles; cell differentiation; developmental genetics; early embryonic stage; gene expression; gene interaction; genetic library; genetic regulation; histogenesis; homeobox genes; in situ hybridization; invertebrate embryology; molecular cloning; molecular genetics; nucleic acid sequence; phenotype; video recording system

The long range goals of this project are to understand the genetic control and molecular mechanisms of pattern formation during development of a cellular embryo, that of the simple and genetically tractable model metazoan Caenorhabditis elegans, by means of a combined genetic and molecular approach. In genetic screens for patterning mutants we have identified five postzygotically expressed nob genes required for posterior patterning of the embryo; at least two of them appear to interact, and one of these is a homologue of the caudal homeobox gene, which is involved in endodermal and posterior development in several diverse organisms. We have also identified a C. elegans TGF-beta homologue similar to the Drosophila decapentaplegic (dpp) gene product, which acts as a morphogen in specification of the dorsal-ventral patterning of embryonic ectoderm as well as in other patterning processes in diverse organisms. In the next project period, we will proceed with 1) investigation of nob gene functions and their interactions, by cloning and molecular characterization of the nob genes, detailed analysis of the corresponding defective phenotypes, and analysis of expression patterns in wild-type and nob mutant backgrounds; 2) continuation of genetic screens for new nob and other patterning genes; 3) investigation of the function of the dpp homologue in C. elegans, by analysis of its expression pattern, isolation of mutants in which it is altered or lacking, and analysis of mutant phenotypes; and 4) investigation of the components and functions of the germ-line-specific P-granules discovered earlier in our laboratory, whose localization in the first four cleavages is likely to be important in specifying embryonic posterior development and the embryonic germ line. The project title, previously "Immunologic studies of C. elegans Early Development," has been changed to better reflect the goals of this research.

该项目的远距离目标是了解遗传控制 以及在开发过程中形成模式形成的分子机制 细胞胚胎，简单和遗传模型的胚胎 秀丽隐杆线虫，通过遗传和 分子方法。在用于图案突变体的遗传筛选中，我们有 鉴定出五个后表达的五个后表达的NOB基因 胚胎的图案；其中至少两个似乎相互作用，一个 其中是尾angox基因的同源物，它参与了 几种不同生物体的内皮和后验发育。我们有 还确定了秀丽隐杆线虫TGF-β同源物类似于果蝇 脱皮链足（DPP）基因产物，它充当形态学 胚胎过胚层的背腹面图案的规范为 以及在各种生物体中的其他图案过程中。在下一个 项目期，我们将继续进行1）调查NOB基因 通过克隆和分子的功能及其相互作用 NOB基因的表征，对相应的详细分析 有缺陷的表型以及野生型表达模式的分析和 Nob突变背景； 2）延续新NOB和 其他图案基因； 3）调查DPP的功能 秀丽隐杆线虫中的同源物，通过分析其表达模式，隔离 突变体被改变或缺乏突变体的分析 表型； 4）调查组件和功能 在我们的实验室早些时候发现的种系特异性的P颗粒 在前四个裂解中的本地化可能很重要 指定胚胎后验发育和胚胎系。 项目名称，以前是“早期对秀丽隐杆线虫的免疫学研究 开发，“已更改为更好地反映目标的目标 研究。