CORE--IMMUNE DEFICIENT MODELS OF CRYPTOSPORIDIOSIS

核心--隐孢子虫病的免疫缺陷模型

• 批准号: 6234975
• 负责人: LANCE E PERRYMAN
• 金额: $ 9.02万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 1997-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6234975
• 关键词: Cryptosporidium; SCID mouse; antimicroorganism antibody; biomedical facility; cooperative study; cryptosporidiosis; disease /disorder model; gastrointestinal infection; immunoglobulin A; immunotherapy; laboratory mouse; life cycle; monoclonal antibody; neutralizing antibody; nonhuman therapy evaluation

This Core is designed to directly support the research objectives of Projects 1,2,3, and 4. Two murine models of persistent infection will be employed. Adult nude mice will be used because of their propensity to develop extra-intestinal crypotsporidial infections, particularly within the biliary duct system. Adult scid mice will also be used because of the ease with which passive antibody transfer and lymphoid cell transplantation protocols can be performed. The specific aims of this Core Component are as follows: 1. Utilize persistently infected nude mice for treatment of extra- intestinal cryptosporidiosis with monoclonal IgA neutralizing antibodies obtained from Project 4. 2. Utilize persistently infected scid mice for evaluation of protective immune responses induced by antigens obtained from Project 3. 3. Test defined antibodies from Projects 1 and 2 for ability to terminate persistent cryptosporidial infection in scid mice.

该核心旨在直接支持以下研究目标 项目 1、2、3 和 4。两种持续感染的小鼠模型将 被雇用。 由于其倾向，将使用成年裸鼠 发生肠外隐孢子虫感染，特别是 胆管系统内。 成年 scid 小鼠也将被使用 因为被动抗体转移和淋巴转移很容易 可以执行细胞移植方案。 具体目标 该核心组件如下： 1.利用持续感染的裸鼠进行治疗 伴有单克隆 IgA 中和抗体的肠道隐孢子虫病 从项目4获得。 2. 利用持续感染的 scid 小鼠进行保护性评估 由项目 3 获得的抗原诱导的免疫反应。 3. 测试项目 1 和 2 中定义的抗体的能力 终止 scid 小鼠的持续性隐孢子虫感染。