CORE--IMMUNE-DEFICIENT MOUSE MODELS OF CRYPTOSPORIDIOSIS

核心--隐孢子虫病免疫缺陷小鼠模型

• 批准号: 3747111
• 负责人: LANCE E PERRYMAN
• 金额: --
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3747111
• 关键词: Cryptosporidium; SCID mouse; antimicroorganism antibody; biliary tract; biomedical facility; cooperative study; cryptosporidiosis; disease /disorder model; immunoglobulin A; immunotherapy; laboratory mouse; life cycle; monoclonal antibody; neutralizing antibody; nonhuman therapy evaluation

This Core is designed to directly support the research objectives of Projects 1,2,3, and 4. Two murine models of persistent infection will be employed. Adult nude mice will be used because of their propensity to develop extra-intestinal crypotsporidial infections, particularly within the biliary duct system. Adult scid mice will also be used because of the ease with which passive antibody transfer and lymphoid cell transplantation protocols can be performed. The specific aims of this Core Component are as follows: 1. Utilize persistently infected nude mice for treatment of extra- intestinal cryptosporidiosis with monoclonal IgA neutralizing antibodies obtained from Project 4. 2. Utilize persistently infected scid mice for evaluation of protective immune responses induced by antigens obtained from Project 3. 3. Test defined antibodies from Projects 1 and 2 for ability to terminate persistent cryptosporidial infection in scid mice.

该核心旨在直接支持 项目1,2,3和4。两个持续感染的鼠模型将 被雇用。 由于它们的倾向，将使用成年裸体小鼠 开发肠外细胞孢子感染，尤其是 在胆道系统中。 成人SCID小鼠也将使用 由于轻松地被动抗体转移和淋巴样 可以执行细胞移植方案。 具体目的 该核心组件如下： 1。利用持续感染的裸鼠治疗外部 肠道孢子虫病与单克隆IgA中和抗体 从项目4获得。 2。利用持续感染的SCID小鼠评估保护性 从项目3获得的抗原引起的免疫反应。 3。项目1和2的测试定义的抗体的能力 在SCID小鼠中终止持续的隐孢子虫感染。