GENETIC ANALYSIS OF TK AND HPRT GENE MUTATIONS

TK 和 HPRT 基因突变的遗传分析

• 批准号: 2311344
• 负责人: MELISSA C LIECHTY
• 金额: $ 12.8万
• 依托单位: APPLIED GENETICS LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-02-17 至 1992-02-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2311344
• 关键词: GENETIC; ANALYSIS; TK; HPRT; GENE

We propose to utilize cytogenetics and molecular genetic techniques to survey the spectrum of mutageneric events that occur at the TK and HPRT loci in mouse L5178Y cells. The purpose of these studies is to determine if the spectrum of genetic abnormalities registered at these loci is a function of the heterozygous/hemizygous nature of the gene loci involved, and to compare the spectrum with the known mutagenic events that occur in humans. This spectrum, for both rare and frequent forms of human cancer, has recently been shown to range from point mutations to loss of large segments of the genome. The demonstration of a similar potential for registering mutagenic damage in in vitro assays is likely to contribute significantly to the understanding of the relationship between in vitro mutagenic potential and human genetic disease. Phase I of this project dealt with the adaptation of recently devised molecular generic technical developments to analysis of mouse L5178Y cells (at the HPRT and TK genes); Phase II will provide a full scale analysis of the hemizygous HPRT gene, and the TK gene in the heterozygous state.

我们建议利用细胞遗传学和分子遗传技术 调查在TK处发生的诱变事件的频谱 小鼠L5178Y细胞中的HPRT基因座。 这些研究的目的 是确定遗传异常的频谱是否已注册 在这些基因座是杂合/半合子性质的函数 涉及的基因基因座，并将光谱与 人类发生的已知诱变事件。 这个频谱，用于 罕见和频繁的人类癌症形式，最近 显示的范围从点突变到损失的大部分 基因组。 演示了类似的潜力 在体外测定中注册诱变损害可能会 为对关系的理解做出了重大贡献 在体外诱变潜力和人类遗传疾病之间。 该项目的第一阶段涉及最近的改编 设计的分子通用技术发展来分析 小鼠L5178Y细胞（在HPRT和TK基因上）；第二阶段 提供半合子HPRT基因的完整分析，并提供 杂合状态的TK基因。