MOUSE AND RAT CHROMOSOME SPECIFIC DNAS AND LIBRARIES

小鼠和大鼠染色体特异性 DNA 和文库

• 批准号: 2157488
• 负责人: MELISSA C LIECHTY
• 金额: $ 36.69万
• 依托单位: APPLIED GENETICS LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-16 至 1997-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2157488
• 关键词: DNA; animal genetic material tag; chromosomes; genetic library; laboratory mouse; laboratory rat; molecular cloning; nucleic acid probes; nucleic acid sequence; oligonucleotides; polymerase chain reaction; southern blotting

DESCRIPTION (Adapted from applicant's abstract): Chromosome-specific DNAs will be prepared for all mouse and rat chromosomes by combining the technologies of chromosome microdissection and degenerate oligonucleotide primed PCR (DOP-PCR) amplification. Difficulties with flow sorting of chromosomes have prevented complete sets of chromosome-specific DNAs or libraries other than human from being made available. Microdissection will circumvent these difficulties and allow pure chromosome-specific DNAs to be prepared from all chromosomes. A single degenerate primer will allow amplification of DNA from both mouse and rat. Cot1 DNA for use as competitor in hybridizations will be prepared for both species. Chromosome-specific DNAs will be tested for specificity by painting. Selected DNAs will be tested for representation by hybridization to a battery of unique chromosome-specific probes. Chromosome-specific DNAs will be cloned using conventional techniques. Chromosome-specific DNAs, Cot1 DNA, and protocols for use will be made available to the research community.

描述（改编自申请人的摘要）：特定于染色体的 DNA将通过组合为所有小鼠和大鼠染色体准备 染色体显微解剖和退化寡核苷酸的技术 启动PCR（DOP-PCR）扩增。流量排序的困难 染色体阻止了完整的染色体特异性DNA或 除人类以外的图书馆被提供。显微解剖 将绕过这些困难，并允许纯染色体特异性 DNA将从所有染色体中制备。单个退化底漆 将允许小鼠和大鼠的DNA扩增。 cot1 DNA 将为这两种物种准备作为杂交的竞争对手。 染色体特异性DNA将通过绘画测试特异性。 选定的DNA将通过杂交与A进行测试以进行表示 独特的特定染色体探针电池。染色体特异性DNA 将使用常规技术克隆。染色体特异性DNA， COT1 DNA和用于使用的协议将用于研究 社区。