NATIONAL TRANSGENIC MOUSE FACILITY

国家转基因小鼠设施

• 批准号: 2314476
• 负责人: MARK SWANSON
• 金额: $ 31.19万
• 依托单位: DNX, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1993-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2314476
• 关键词: NATIONAL; TRANSGENIC; MOUSE; FACILITY

The NICHD will provide, through the contract mechanism, a facility for supplying transgenic mice to the scientific community. The demand for transgenic mice is substantial, but the ability to produce them is limited. Thus, the aim of this contract is to make available to the general scientific community at large access to the technology necessary for the production of transgenic mice. More specifically, the Contractor shall be responsible for the following: a. accepting donor DNA probes either as linear DNA, or in a cloning vector but not as bacteria containing plasmid. b. confirming the identity of the donor DNA. c. generating by microinjection techniques transgenic mice using the DNA insert provided by the donor. d. confirming integration of donor DNA into the genome of the mice produced. e. maintaining the transgenic mice at the facility and distributing the mice to individuals in the scientific community who have requested the service. f. instituting a program to publicize the availability of this service.

NICHD 将通过合同机制，为 向科学界提供转基因小鼠。 需求 转基因小鼠数量巨大，但生产它们的能力有限。 因此，本合同的目的是向一般人提供 科学界普遍获得必要的技术 转基因小鼠的生产。 更具体地说，承包商应负责以下事项： 一个。 接受线性 DNA 或克隆载体中的供体 DNA 探针 但不像含有质粒的细菌。 b. 确认供体 DNA 的身份。 c. 使用DNA通过显微注射技术产生转基因小鼠 插入物由捐赠者提供。 d. 确认供体 DNA 整合到小鼠基因组中 产生的。 e. 在该设施中饲养转基因小鼠并分发 老鼠给科学界中那些要求的个人 服务。 f. 制定一项计划来宣传该服务的可用性。