Microscopy and Image Analysis Core

显微镜和图像分析核心

• 批准号: 10557025
• 负责人: Robert T Wheeler
• 金额: $ 42.64万
• 依托单位: UNIVERSITY OF MAINE ORONO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-05 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10557025
• 关键词: Address; Affect; Aging; Architecture; Biochemistry; Biological; Biology; Biomedical Engineering; Biomedical Research; Cells; Cellular Structures; Centers of Research Excellence; Chemical Engineering; Communicable Diseases; Communities; Computer software; Core Facility; Cues; Data; Data Storage and Retrieval; Engineering; Equipment; Experimental Designs; Fluorescence; Funding; Future; Goals; Grant; Head; Image; Image Analysis; Immune; Immune response; Infection; Infrastructure; Institution; Instruction; Island; Label; Light; Maine; Mentorship; Microscope; Microscopy; Modeling; Molecular; Morphogenesis; Muscle; Optics; Outcome; Output; Partner in relationship; Physics; Proteins; Publications; Reproducibility; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Science; Scientist; Services; Signal Transduction; Specialist; Stimulus; Structure; System; Time; Tissues; Training; Translating; Translation Process; United States National Institutes of Health; Universities; Viral; Visualization; Work; Zebrafish; biological systems; cell behavior; cell growth regulation; cell motility; data management; design; early-career faculty; empowerment; environmental change; experience; experimental study; extracellular; improved; in vivo; innovation; longitudinal design; migration; novel; open source; programs; repaired; response; theories; tool; two-photon; ultra high resolution

ABSTRACT: MICROSCOPY AND IMAGE ANALYSIS CORE The proposed COBRE will establish a center of excellence at the University of Maine (UMaine) focusing on the impacts of extracellular signaling on cell behavior. The five interconnected projects in this proposal will elucidate the intricacies of extracellular signaling, which will require sophisticated and dynamic analyses of cellular structure in vivo. In addition, all five of the proposed research projects are structured around longitudinal designs that require higher throughput imaging of live systems over a longer period of time. The five projects will also incorporate novel, unbiased image analysis paradigms that will increase rigor and reproducibility. To address these needs, the proposed COBRE Microscopy and Image Analysis Core (MIAC) has two specific aims to provide training and access to (1) state-of-the art, high resolution live imaging services and (2) experiment planning, image acquisition and image analysis services as well as data storage and management. MIAC will provide training and access to state-of-the-art, high resolution live imaging services, including a recently-acquired inverted Leica confocal/lightsheet microscope, an upright Zeiss Airyscan2, and an Olympus FV-1000 confocal. In addition, funding through the NIH COBRE program will allow UMaine to acquire an inverted Zeiss LSM900 with Airyscan2 confocal microscope designed for live imaging of infectious disease, which will be a unique capacity in the state. MIAC will provide wraparound services from experimental planning to expert data management and image analysis. These services are critical for rigor, reproducibility and maximizing the impact of microscopy equipment on the proposed research projects and for other users. The MIAC core will provide one-on-one mentorship of users in planning, executing, analyzing, and storing their data. This will include instruction in the theory and practice of microscopy, as well as ongoing support for microscope troubleshooting, image analysis with Zen, ImageJ, Matlab, Imaris and Aivia software packages. The MIAC core is innovative in that it will leverage existing engineering, physics and biochemistry expertise in imaging and analysis to guide projects and exploit the power of our microscopes and analysis software from start to finish. The establishment of a Microscopy and Image Analysis Core at UMaine will fulfill important gaps in biomedical infrastructure in the state and catalyze important new discoveries in how cell behavior is governed by extracellular signaling in multiple contexts.

摘要：显微镜和图像分析核心 拟议的 COBRE 将在缅因大学 (UMaine) 建立一个卓越中心，重点关注 细胞外信号传导对细胞行为的影响。该提案中的五个相互关联的项目将 阐明细胞外信号传导的复杂性，这将需要复杂和动态的分析 体内细胞结构。此外，所有五个拟议的研究项目都是围绕 纵向设计，需要在较长时间内对实时系统进行更高吞吐量的成像。这 五个项目还将纳入新颖、公正的图像分析范式，这将提高严谨性和 再现性。为了满足这些需求，提出了 COBRE 显微镜和图像分析核心 (MIAC) 有两个具体目标，即提供培训和获得 (1) 最先进的高分辨率实时成像服务 (2) 实验规划、图像采集和图像分析服务以及数据存储和 管理。 MIAC 将提供培训并提供最先进的高分辨率实时成像 服务，包括最近购买的倒置徕卡共焦/光片显微镜、正置蔡司 Airyscan2 和 Olympus FV-1000 共焦。此外，通过 NIH COBRE 计划提供的资金将允许 缅因大学购买倒置蔡司 LSM900 和 Airyscan2 共焦显微镜，专为实时成像而设计 传染病，这将是该州独特的能力。 MIAC 将提供全方位服务 实验规划到专家数据管理和图像分析。这些服务对于 严谨性、可重复性以及最大限度地提高显微镜设备对拟议研究项目的影响 以及其他用户。 MIAC 核心将为用户提供一对一的指导，包括规划、执行、 分析和存储他们的数据。这将包括显微镜理论和实践的指导，以及 作为对显微镜故障排除、使用 Zen、ImageJ、Matlab、Imaris 和 Aivia 进行图像分析的持续支持 软件包。 MIAC 核心的创新之处在于它将利用现有的工程、物理和 成像和分析方面的生物化学专业知识可指导项目并利用我们显微镜的力量 分析软件从头到尾。在缅因大学建立显微镜和图像分析核心 将填补该州生物医学基础设施的重要空白，并促进重要的新发现 细胞行为在多种情况下受到细胞外信号的控制。