How can mosquitoes develop and reproduce in the complete absence of juvenile hormone?

在完全没有保幼激素的情况下，蚊子如何发育和繁殖？

基本信息

批准号：
10554310
负责人：
Francisco Fernandez-Lima
金额：
$ 14.75万
依托单位：
FLORIDA INTERNATIONAL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-01-24 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10554310
关键词：
Acids Address Adult Aedes Affect Area Basic Science Biological Biological Metamorphosis Biology CRISPR/Cas technology Chikungunya fever Complex Crustacea Culicidae Cytochrome P450 Dengue Dengue Fever Development Ecosystem Endocrine Endocrine Disruptors Enzymes Event Evolution Experimental Models Female Future Genes Goals Homeostasis Hormonal Incidence Insect Control Insecta Insecticides Invaded Juvenile Hormones Knowledge Larva Libraries Life Cycle Stages Malaria Mediating Messenger RNA Methoprene Modeling Molecular Mosquito Control Mosquito-borne infectious disease Nutritional Output Ovary Pathway interactions Performance Physiological Play Process Proteins Public Health RNA Interference Regulatory Pathway Reproduction Reproductive Biology Research Resistance Risk Role Signal Transduction Testing Transferase Validation Work ZIKA base chikungunya cost design differential expression epoxidase experience experimental study fitness gene network hormonal signals hormone analog hormone biosynthesis improved innovation life history methyl farnesoate molecular recognition mosquito reproduction mutant novel strategies public health relevance receptor reproductive reproductive fitness sex transcriptome transcriptomics vector vector control zika fever

项目摘要

PROJECT SUMMARY Recent dramatic increases in the incidence of mosquito-borne diseases, like Malaria, Zika, Chikungunya and Dengue Fever, and the wide-spread resistance of mosquitoes to insecticides emphasizes the need for new approaches for insect control based on mosquito-specific agents. The discovery of such mosquito-specific control agents depends on continued basic research on the biology of mosquitoes. Juvenile hormone (JH), an epoxidated sesquiterpenoid, is an essential regulator of major developmental and life history events in insects. Insects and crustaceans originated from aquatic pancrustacean ancestors that invaded terrestrial ecosystems over 450 million years ago. The Methoprene-tolerant (Met) protein is the intracellular receptor for insect JHs. The ability of Met to respond to MF, the “crustacean JH”, suggests that the role of Met in sesquiterpenoid signaling precedes the evolutionary separation of the hexapoda from the crustacean lineages. To address the evolutionary and biological significance of MF epoxidation, we generated mosquitoes completely lacking either of the two enzymes that catalyze the last steps of MF/JH biosynthesis and epoxidation, the methyl transferase (JHAMT) and the P450 epoxidase CYP15 (EPOX). jhamt-/- larvae lacking both MF and JH died at the onset of metamorphosis. While epox-/- mutants, which synthesized MF but no JH completed the entire life cycle. While epox-/- adults are fertile, the reproductive performance of both sexes is dramatically reduced. Using these two lines of null mutant mosquitoes, we will investigate how in the absence of JH, MF is sufficient to complete adult development. Our hypothesis is MF is able to properly activate the gene networks that control mosquito larval development and metamorphosis. Additionally, we will investigate the reproductive fitness cost in JH null females. We expect to recognize the molecular mechanism underlying the differences in JH signaling in our three model lines (jhamt-/-, epox-/- and WT). Our two null mutant mosquito lines provide a unique opportunity to explore the role of non-epoxidated versus epoxidated JHs in the development and reproduction of insects. Completing the proposed research could lead to the identification of targets for designing new, specific and affordable strategies suitable for mosquito control.

项目概要最近疟疾、寨卡病毒、基孔肯雅热和登革热等蚊媒疾病的发病率急剧增加发烧和蚊子对杀虫剂的广泛耐药性强调需要新的昆虫防治方法基于蚊子特异性防治剂的控制这种蚊子特异性防治剂的发现取决于持续不断的研究。保幼激素（JH）是一种环氧化倍半萜类化合物，对蚊子生物学的基础研究至关重要。昆虫和甲壳类动物主要发育和生活史事件的调节者，起源于水生动物。 4.5 亿多年前入侵陆地生态系统的泛甲壳动物祖先。蛋白质是昆虫 JH 的细胞内受体 Met 对 MF（“甲壳动物 JH”）作出反应的能力表明： Met 在倍半萜类信号传导中的作用早于六足动物与甲壳类动物的进化分离为了解决 MF 环氧化的进化和生物学意义，我们完全产生了蚊子。缺乏催化 MF/JH 生物合成和环氧化最后步骤的两种酶中的任何一种，甲基缺乏 MF 和 JH 的幼虫在发病时死亡。 While epox-/-突变体合成了MF但没有JH完成了整个生命周期。成虫具有生育能力，但使用这两个无效突变体系，两性的繁殖性能均显着降低。对于蚊子，我们将研究在没有 JH 的情况下，MF 如何足以完成我们的假设。 MF 能够正确激活控制蚊子幼虫发育和变态的基因网络。此外，我们将研究 JH 无效雌性的生殖健康成本，我们希望识别该分子。我们的三个模型系（jhamt-/-、epox-/- 和 WT）中 JH 信号传导差异的机制。突变蚊子系提供了一个独特的机会来探索非环氧化 JH 与环氧化 JH 在完成拟议的研究可以确定昆虫的目标。设计新的、具体的和负担得起的适合蚊子控制的策略。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effects of mating on female reproductive physiology in the insect model, Rhodnius prolixus, a vector of the causative parasite of Chagas disease.

交配对昆虫模型中雌性生殖生理的影响，Rhodnius prolixus，恰加斯病的致病寄生虫的载体。

DOI：
发表时间：
2023-09
期刊：
影响因子：
3.8
作者：
Leyria, Jimena;Guarneri, Alessandra A;Lorenzo, Marcelo G;Nouzova, Marcela;Noriega, Fernando G;Benrabaa, Samiha A M;Fernandez;Valadares Tose, Lilian;Orchard, Ian;Lange, Angela B
通讯作者：
Lange, Angela B

The embryonic role of juvenile hormone in the firebrat, Thermobia domestica, reveals its function before its involvement in metamorphosis.

保幼激素在火鳟（Thermobia Domestica）中的胚胎作用揭示了它在参与变态之前的功能。

DOI：
发表时间：
2024-01-18
期刊：
影响因子：
0
作者：
Truman, James W;Riddiford, Lynn M;Konopová, Barbora;Nouzova, Marcela;Noriega, Fernando G;Herko, Michelle
通讯作者：
Herko, Michelle

Differential gene expression and microRNA profile in corpora allata-corpora cardiaca of Aedes aegypti mosquitoes with weak juvenile hormone signalling.

具有弱保幼激素信号传导的埃及伊蚊的全体-贲门体中的差异基因表达和 microRNA 谱。