PRECHRONIC & CHRONIC TOXICITY OF EMODIN

前纪

• 批准号: 2311021
• 负责人: J D PREJEAN
• 金额: $ 40万
• 依托单位: SOUTHERN RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-03-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2311021
• 关键词: PRECHRONIC; CHRONIC; TOXICITY; EMODIN

The specific objectives of this investigation is to evaluate the prechronic and chronic toxicity and carcinogenicity of emodin in Fischer-344 rats and B6C3F1 mice. The following studies will be performed: Fourteen-Day Repeated-Dose Prechronic Toxicity Study: This study will characterize the toxicity associated with dietary exposure to emodin including the identification of target organs, determination of a no-effect level (if feasible), differences in sensitivity between sexes and species, and the slope of the dose-response curve. Thirteen-Week (90-Day) Subchronic Study: In addition to obtaining toxicological data as described under the 14-day repeated-dose toxicity study, this study will also help to determine the doses for each strain and species in the chronic 2-year toxicity study. This investigation includes necropsy and histopathologic evaluations, preparation of unstained blood smears (for use by the NTP in micronuleic determinations) and determination of blood concentrations of emodin in experimental animals. Two-Year Chronic Study: The two-year study will evaluate the chronic toxicity and carcinogenicity of emodin administered to male and female F344 rats and. B6C3F1 mice. All moribund animals, as well as those that survive to the end of the study, will receive a complete gross examination. All grossly visible lesions and all tissues with non-neoplastic or neoplastic lesions will receive a complete histopathologic examination.

这项调查的具体目标是评估 EMODIN的毒性和慢性毒性和慢性毒性 Fischer-344大鼠和B6C3F1小鼠。 以下研究将是 执行： 十四天重复剂量的预毒性毒性研究：这项研究 将表征与饮食暴露有关的毒性 EMODIN包括鉴定目标器官，确定 无效水平（如果可行），灵敏度差异 在性别和物种之间以及剂量反应的斜率 曲线。 13周（90天）亚慢性研究：除了获得 如14天重复剂量所述的毒理学数据 毒性研究，这项研究还将有助于确定剂量 对于慢性2年毒性研究中的每个菌株和物种。 该研究包括尸检和组织病理学 评估，准备未染上的血液涂片（供 NTP在微观测定中）和血液的测定 实验动物中的Emodin浓度。 为期两年的慢性研究：两年的研究将评估 对雄性的Emodin的慢性毒性和致癌性 和雌性F344大鼠。 B6C3F1小鼠。 所有垂死动物，如 以及那些生存到研究结束的人，将获得 完整的总考试。 所有严重可见的病变和所有 具有非塑性或肿瘤病变的组织将接受 完整的组织病理检查。