Center for multidimensional atlas of the human heart

人类心脏多维图谱中心

• 批准号: 10530968
• 负责人: Jeffrey Moffitt
• 金额: $ 221.18万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10530968
• 关键词: ATAC-seq; Adult; Age; Aging; Algorithms; Anatomy; Atlases; Automobile Driving; Awareness; Basic Science; Biological Assay; Biology; Biomedical Research; Boston; Cell Communication; Cell Nucleus; Cells; Cellular Structures; Childhood; Chromatin; Clinical Data; Clinical Research; Collaborations; Communities; Complex; Computational algorithm; Computer Models; Computer software; Computing Methodologies; Data; Data Analyses; Databases; Detection; Development; Diagnosis; Disease; Emerging Technologies; Ethnic Origin; Fluorescent in Situ Hybridization; Follow-Up Studies; Freezing; Genetic Transcription; Health; Heart; Heart Diseases; Heart Research; Heterogeneity; Human; Human BioMolecular Atlas Program; Image; Imaging technology; Informed Consent; Infrastructure; Iowa; Knowledge; Longevity; Maps; Mediating; Medical; Methods; Molecular; Molecular Profiling; Normalcy; Organ; Outcome; Pattern; Pediatric Hospitals; Pennsylvania; Phase; Phenotype; Philadelphia; Physiological; Population; Production; Prognosis; Proteins; Proteomics; Protocols documentation; RNA; Regulatory Pathway; Research; Research Personnel; Signal Pathway; Signal Transduction Pathway; Small Nuclear RNA; Spatial Distribution; Specimen; Techniques; Technology; Tissue Donors; Tissue Preservation; Tissue Procurements; Tissues; Translational Research; Universities; Visualization; XCL1 gene; base; biobank; burden of illness; cell type; cellular imaging; complex data; computational pipelines; computerized tools; data sharing; epidemiologic data; epigenomics; genomic data; human disease; human tissue; improved; indexing; molecular imaging; multiple omics; multiplexed imaging; open source; precision medicine; searchable database; sex; single cell analysis; tissue mapping; transcriptomics; user-friendly

PROJECT SUMMARY - OVERALL The human heart presents remarkable anatomical, cellular and functional heterogeneity, with specialized cellular structures performing distinct yet essential physiological functions. A significant gap of knowledge is that different cells’ molecular signature, spatial distribution and interactions, and functional state remain little understood for the heart. We therefore propose the in-depth characterization of the heart which accounts for a large fraction of human disease burden. We will map molecular and cellular changes in heart tissues over the course of human lifespan using comprehensive multi-dimensional single-cell and imaging technologies. The final product will impact research of the heart in the following manner: 1) Organ Atlases: spatially resolved atlases will provide a highly user friendly, publicly available, searchable database of the most comprehensive multi-omic, single cell analysis of the heart. Molecular data will be richly annotated with additional clinical and epidemiological data. 2) Computational methods: in addition to the data, the critical computational tools and pipelines developed in this project will be available to the research community. These include methods and pipelines for processing multi-omics and imaging data, inference of cell-specific regulatory and signaling pathways, correlation of mesoscale imaging and molecular imaging features, as well as database algorithms for the query, exploration and visualization of highly complex data. 3) Access to biospecimens for follow-up studies: biospecimens collected in this project will be banked and made available to the biomedical research community. These include freshly frozen and fixed specimens and tissue sections. In summary, the proposed project will broadly impact the entire research community and jumpstart basic-science and medical discoveries based on a sophisticated understanding of the key molecular circuits underlying the development and aging of the heart.

项目概要 - 总体 人类心脏呈现出显着的解剖学、细胞和功能异质性，具有专门的 细胞结构执行独特但重要的生理功能，这是一个重大的知识空白。 不同细胞的分子特征、空间分布和相互作用以及功能状态仍然很少 因此，我们建议对心脏进行深入的表征。 我们将绘制心脏组织的分子和细胞变化图。 利用全面的多维单细胞和成像技术来研究人类生命的过程。 最终产品将以以下方式影响心脏研究：1）器官图谱：空间解析 地图集将提供一个高度用户友好、公开可用、可搜索的最全面的数据库 心脏的多组学、单细胞分析将通过额外的临床和数据进行丰富的注释。 2）流行病学数据：除了数据之外，关键的计算工具和 该项目开发的管道将可供研究界使用，其中包括方法和方法。 用于处理多组学和成像数据、细胞特异性调控和信号传导的推断的管道 途径、介观成像和分子成像特征的相关性以及数据库算法 用于高度复杂数据的查询、探索和可视化 3) 获取生物样本以进行后续跟踪。 研究：该项目中收集的生物样本将被存储并可供生物医学研究使用 这些包括新鲜冷冻和固定的标本和组织切片。 该项目将广泛影响整个研究界并推动基础科学和医学发现 基于对发育和老化的关键分子电路的深入理解 心脏。