PEPTIDERGIC MODULATION OF SYMPATHETIC COTRANSMISSION

交感神经传导的肽调节

基本信息

批准号：
2293053
负责人：
Violeta N. Mutafova-Yambolieva
金额：
$ 3.65万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-09-20 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/2293053
关键词：
PEPTIDERGIC MODULATION SYMPATHETIC COTRANSMISSION

项目摘要

The proposed research during the fellowship will provide me with vital experience of sympathetic nervous system activity studies. Thus, my main goals for this period are: (i) To study the modulating effects of cardiovascular peptides ET, ANP, and BNP on the pre- and postsynaptic mechanisms of peripheral sympathetic cotransmission at the levels of isolated tissues in vitro and of an organism devoided of central reflexes in vivo as well as to study the participation of the endothelium, the EDRF/NO, cGMP, IP3, and Ca++ in these effects; (ii) To learn the basic techniques necessary for studies on peripheral sympathetic cotransmission, particularly those related to a) transmitter (ATP and NE) release measurement, b) contractile responses in isolated blood vessels, c) intracellular Ca++ and cyclic nucleotide signaling and d) hemodynamics of pitched animals (see Item 21, Research plan). Professor Westfall's laboratory is an ideal place to study the role of endogenous peptides on sympathetic neurotransmission. Professor David Westfall and his group have played a fundamental role in developing the concept that sympathetic neurotransmission involves the release of the cotransmitters ATP and NE. In addition his group has recently developed the concept that prejunctional purinoceptors, which modulate neurotransmitter release, may exist as several subtype. The laboratory is also well known for its ability to develop new methodologies to apply to issues of neurotransmission. For example, his laboratory is one of the few in the world where the release of ATP and NE can be simultaneously evaluated. The opportunity to learn and work in such an environment particularly in a place where the research interests are so congruent with my own, will no doubt enhance the development of my scientific career.

奖学金期间拟议的研究将为我提供至关重要的交感神经系统活动研究的经验。因此，我的主要人物此期间的目标是：（i）研究前后突触的心血管肽ET，ANP和BNP 外周外共转移的机制在分离的组织在体外和有机体的中央反射体内以及研究内皮的参与这些效果中的EDRF/NO，CGMP，IP3和Ca ++；（ii）学习基本研究周围交感神经所需的技术共转移，特别是与a）发射器（ATP和 NE）释放测量，b）孤立血液中的收缩反应血管，c）细胞内Ca ++和环状核苷酸信号传导和 d）俯仰动物的血液动力学（请参阅第21项，研究计划）。 Westfall教授的实验室是研究角色的理想场所内源性肽在交感神经传递上。大卫教授 Westfall及其小组在发展中发挥了基本作用同情神经传递的概念涉及释放共晶质体ATP和NE。此外，他的小组最近发展了持续的purinoceptors的概念调节神经递质的释放可能以几种亚型存在。实验室还以开发新方法的能力而闻名到神经传递的问题。例如，他的实验室是在世界上释放ATP和NE的少数同时评估。学习和工作的机会环境特别是在研究兴趣的地方与我自己的一致，无疑会增强我的发展科学职业。