NEUROMUSCULAR TRANSMISSION IN ARTERIES AND VEINS

动脉和静脉中的神经肌肉传输

• 批准号: 6330156
• 负责人: Violeta N. Mutafova-Yambolieva
• 金额: $ 19.99万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-10 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6330156
• 关键词: adenosine triphosphate; alpha adrenergic receptor; blood vessels; gastrointestinal circulation; guinea pigs; high performance liquid chromatography; membrane potentials; microelectrodes; neuromuscular transmission; neurotransmitter receptor; neurotransmitter transport; norepinephrine; purinergic receptor; vascular resistance; vascular smooth muscle; vascular smooth muscle nervous control

DESCRIPTION: (adapted from the applicant's abstract) The long range objective of the research outlined in this proposal is to characterize neural control mechanisms of resistance and capacitance blood vessels of the splanchnic circulation and determine mechanisms which underlie differential regulation of these two vascular networks. The proposed studies will compare sympathetic co-transmission in guinea pig mesenteric artery and vein. The PI hypothesizes that responses to nerve stimulation in vein and artery differ because: (1) the mechanisms which are initiated by ATP and norepinephrine (NE) in smooth muscle differ and/or (2) the amount and/or ratio of ATP and NE at the vicinity of the postjunctional receptor differ. Specific Aims 1 and 2 will address the first part of this hypothesis by characterizing the effects of exogenous and endogenous NE and ATP on membrane potential (intracellular microelectrode techniques) and force development (organ bath experiments) in artery and vein and determining whether different subtypes of P2 purinergic and alpha-adrenergic receptors are involved. Specific Aim 3 will address the second part of this hypothesis by employing state-of-the-art techniques to simultaneously measure nerve evoked outflow of ATP (HPLC-fluorscence detection) and NE (HPLC- electrochemical detection) in artery and vein. Thus, mesenteric artery and vein will be uniquely compared in terms of both transmitter release and postjuctional effector mechanisms. These studies will provide new insights into the differential control of arteries and veins by nerves. They will also aid in clarifying the role of ATP as a neurotransmitter in the splanchnic circulation and its relationship to NE as the primary sympathetic neurotransmitter.

描述：（改编自申请人的摘要）远程 该提案中概述的研究的目的是表征 抗药性和电容血管的神经控制机制 斑点循环并确定基础的机制 这两个血管网络的差异调节。提议 研究将比较豚鼠中的交感神经传播 肠系膜动脉和静脉。 PI假设对神经的反应 静脉和动脉的刺激不同是因为：（1） 由平滑肌中的ATP和去甲肾上腺素（NE）引发 和/或（2）ATP和NE在附近的ATP和/或比率 术后受体不同。具体目标1和2将解决 通过表征外源性的影响，该假设的第一部分 以及膜电位上的内源性NE和ATP（细胞内 微电极技术）和力开发（器官浴 实验）在动脉和静脉中，并确定是否不同 P2嘌呤能和α-肾上腺素能受体的亚型涉及。 具体目标3将通过 采用最先进的技术同时测量神经 诱发ATP的流出（HPLC-荧光检测）和NE（HPLC- 电化学检测）在动脉和静脉中。因此，肠系膜动脉 和静脉将在两个发射器释放方面进行唯一比较 和后效应器机制。这些研究将提供新的 通过神经对动脉和静脉的差异控制的见解。 他们还将有助于阐明ATP作为神经递质的作用 在闪存的流通及其与NE作为主要的关系中 交感神经递质。