CORE--PREPARATION, SUPPLY OF TOXOPLASMA AND IMMUNOLOGICALS

核心——弓形虫和免疫制剂的制备、供应

• 批准号: 5205425
• 负责人: FAUSTO ARAUJO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5205425
• 关键词: Toxoplasma gondii; antimicroorganism antibody; biomedical facility; immunological substance; interferon gamma; laboratory rabbit; life cycle; protozoal antigen; protozoal cyst; tissue /cell culture

In the present grant proposal the individual projects are designed to examine the activity of new therapeutic agents against T. gondii, to investigate new enzymatic pathways that may prove important for design of drugs active against the parasite and to identify and characterize the genes of the organism that are responsible for drug resistance. The ultimate objectives are to identify more active and safer therapeutic agents for treatment of toxoplasmosis, particularly toxoplasmic encephalitis in AIDS patients. For each of the proposed studies, tachyzoites, cysts and bradyzoites of different strains of T. gondii will be needed in large numbers. Tachyzoites and cysts will be used for infection of mice and of mammalian cells in tissue culture for in vivo and in vitro evaluation of the activity of therapeutic agents against T. gondii. Tachyzoite and bradyzoites will also be used to investigate biochemical and metabolic pathways which may prove to be useful as targets for inactivation or inhibition by therapeutic agents. These forms of T. gondii will be provided by this Core. In addition, this Core will also provide antisera to T. gondii for use in serological and immunochemical assays and antisera to IFN-gamma for use un preparation of tachyzoites of strains of T. gondii of low virulence for mice.

在本拨款提案中，各个项目旨在 检查针对弓形虫的新治疗剂的活性，以 研究可能对设计很重要的新酶途径 药物对寄生虫有活性，并识别和表征 负责耐药性的生物体基因。 这 最终目标是找到更积极、更安全的治疗方法 用于治疗弓形虫病，特别是弓形虫病的药剂 艾滋病患者的脑炎。 对于每项拟议的研究， 不同弓形虫菌株的速殖子、包囊和缓殖子将 需要大量。 速殖子和包囊将用于 小鼠和组织培养中哺乳动物细胞的体内感染 以及体外评估治疗剂针对 T 的活性。 弓形虫。 速殖子和缓殖子也将用于研究 生化和代谢途径可能被证明是有用的 治疗剂失活或抑制的靶标。 这些 该核心将提供弓形虫的各种形式。 另外，这个核心 还将提供弓形虫抗血清，用于血清学和 免疫化学测定和 IFN-γ 抗血清用于制备 对小鼠低毒力弓形虫菌株的速殖子。