RETROVIRUSES--RECOMBINATION/RETROFECTION/PATHOGENESIS

逆转录病毒——重组/逆转录/发病机制

• 批准号: 2281022
• 负责人: ADELINE M HAJJAR
• 金额: $ 6.13万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2281022
• 关键词: Retroviridae; Retroviridae disease; cats; feline immunodeficiency virus; genetic recombination; genetic transcription; helper virus; molecular biology; nucleic acid sequence; polymerase chain reaction; restriction mapping; southern blotting; virus RNA; virus cytopathogenic effect; virus genetics

The goal of this study is to obtain training and expertise in the molecular biology of retroviruses with the eventual application of such experience to the study of the pathogenesis of feline immunodeficiency virus (FIV). Phase I of this plan is devoted to learning the various techniques and approaches used in molecular virology while studying avian retroviruses. During this initial phase, experience will be obtained in tissue culture as well as DNA, RNA, and protein manipulations. This phase will focus on retrofection, or the ability of retroviral particles to transmit desired phenotypes to recipient cells by infection, and on heterologous recombination which is recombination between retroviral and non-retroviral sequences. Part of this research will be used to fulfill thesis requirements for a Ph.D. degree in Microbiology. Phase II of this program will involve application Of the molecular approaches and techniques learned in Phase I to the study of FIV tropism and pathogenesis. FIV is a recently discovered lentivirus that causes immunodeficiency disease in cats. It is also a good animal model for AIDS. Molecular clones of FIV will be obtained directly from cat tissues and characterized in vitro during Phase II. Future experiments in cats will evolve from these studies and will be the focus of future grant applications. The studies in Phases I and II will be carried out under the supervision of well-established individuals in the field of molecular retrovirology.

本研究的目的是获得以下方面的培训和专业知识 逆转录病毒的分子生物学及其最终应用 猫免疫缺陷发病机制研究经验 病毒（FIV）。 该计划的第一阶段致力于学习各种 研究禽类时用于分子病毒学的技术和方法 逆转录病毒。 在此初始阶段，将获得以下方面的经验： 组织培养以及 DNA、RNA 和蛋白质操作。 这 该阶段将重点关注逆转录或逆转录病毒颗粒的能力 通过感染将所需的表型传递给受体细胞，然后 异源重组是逆转录病毒和逆转录病毒之间的重组 非逆转录病毒序列。 这项研究的一部分将用于实现 博士学位论文要求微生物学学位。 本次第二期 计划将涉及分子方法的应用和 在第一阶段学到的技术来研究 FIV 趋向性和 发病。 FIV 是最近发现的一种慢病毒，可导致 猫的免疫缺陷疾病。 这也是一个很好的动物模型 艾滋病。 FIV的分子克隆将直接从猫组织中获得 并在第二阶段进行体外表征。 未来在猫身上进行的实验 将从这些研究中发展而来，并将成为未来资助的重点 应用程序。 第一阶段和第二阶段的研究将在 对分子领域知名人士的监督 逆转录病毒学。