TYPE 1 VS TYPE 2 CYTOKINE RESPONSE DURING FIV INFECTION

FIV 感染期间 1 型与 2 型细胞因子反应

• 批准号: 2002692
• 负责人: PAUL R AVERY
• 金额: $ 6.17万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-03-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2002692
• 关键词: Retroviridae disease; cats; cytokine; feline immunodeficiency virus; humoral immunity; in situ hybridization; interleukin 12; microorganism disease chemotherapy; microorganism immunology; nonhuman therapy evaluation; polymerase chain reaction; tissue /cell culture

DESCRIPTION: (Adapted from the application abstract) Using a strain of feline immunodeficiency virus (FIV) that induces rapid disease, the applicant will examine whether a cytokine profile switch from a Type 1 to a Type 2 response occurs during progression to AIDS and, if so, how this change relates to the immunopathology associated with the virus. Aim 1 will focus on the patterns of cytokine production (Type 1 versus Type 2) from the time of initial viral replication through progression to clinical illness. Aim 2 will use the model developed in Aim 1 to study the effect of early treatment with IL-12, a potent Type 1 cytokine in the host's ability to control viral replication and resist disease induction. a. Candidate: Dr. Avery received his VMD in 1991 from Cornell University. He worked for 2 years in practice, then worked as a Research Fellow in Dr. Finberg's laboratory. Dr. Avery lists one publication from work in Dr. Finberg's laboratory and a manuscript in preparation. b. Mentor: Dr. Edward Hoover is a Professor in the Department of Pathology at Colorado State University. He has mentored 18 Ph.D. and postdoctoral students. His laboratory has developed the key systems that will be used in this project. Dr. Hoover has R01 and N01 grants to study FIV pathogenesis and to use the FIV model for evaluation of therapeutic approaches. c. Career Development Plan: Coursework is included in the career development plan which will provide the candidate with the background in virology, molecular biology, biochemistry and immunology that will be necessary for this project and for a career in infectious disease/AIDS research. d. Research Plan: Studies are proposed to utilize an accelerated FIV/cat infection model employing a highly virulent strain of virus which induces rapid immunodeficiency disease and a corresponding high viral burden. This rapid disease progression model will be used to examine potential perturbances in the patterns of TH1-like and TH2-like cytokine expression which may accompany infection and progression to a fatal outcome. An Advisory Committee is available to guide and review the candidate's research progress. e. Environment and Institutional Commitment: The proposed research will be performed in Dr. Hoover's laboratory in the Department of Pathology at Colorado State University.

描述：（改编自应用摘要）使用 猫科动物免疫缺陷病毒（FIV）可诱发快速疾病， 申请人将检查细胞因子谱是否从 1 型转变为 1 型 2 型反应发生在艾滋病进展过程中，如果是的话，这是如何发生的 变化与病毒相关的免疫病理学有关。 目标1将 重点关注细胞因子产生的模式（1 型与 2 型） 从最初病毒复制到发展为临床疾病的时间。 目标 2 将使用目标 1 中开发的模型来研究早期的影响 使用 IL-12 治疗，IL-12 是一种有效的 1 型细胞因子，可增强宿主的能力 控制病毒复制并抵抗疾病诱导。 一个。候选人：Avery 博士于 1991 年从康奈尔大学获得 VMD。 他在实践中工作了 2 年，然后在 Dr. 担任研究员。 芬伯格的实验室。 艾弗里博士列出了《博士》中的一篇出版物。 芬伯格的实验室和正在准备的手稿。 b.导师：Edward Hoover 博士，病理学系教授 在科罗拉多州立大学。 他已指导了18名博士生。和博士后 学生。 他的实验室开发了关键系统，将用于 这个项目。 Hoover 博士拥有 R01 和 N01 拨款来研究 FIV 发病机制 并使用 FIV 模型来评估治疗方法。 c.职业发展计划：课程作业包含在职业生涯中 发展计划将为候选人提供背景 病毒学、分子生物学、生物化学和免疫学 对于这个项目以及传染病/艾滋病领域的职业来说是必要的 研究。 d.研究计划：建议研究利用加速 FIV/cat 采用高毒力病毒株的感染模型，可诱导 快速免疫缺陷病和相应的高病毒负荷。 这 快速疾病进展模型将用于检查潜在的 TH1 样和 TH2 样细胞因子表达模式的扰动 这可能会伴随感染并进展到致命的结果。 一个 咨询委员会可指导和审查候选人的研究 进步。 e.环境和机构承诺：拟议的研究将 在病理科 Hoover 博士的实验室进行 科罗拉多州立大学。