High Frequency Wearable and Transparent Electrostrictive Row-Column Arrays for Whole Brain Functional Imaging

用于全脑功能成像的高频可穿戴透明电致伸缩行列阵列

• 批准号: 10489845
• 负责人: Roger J Zemp
• 金额: $ 16.2万
• 依托单位: UNIVERSITY OF ALBERTA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10489845
• 关键词: 3-Dimensional; 3D ultrasound; Acoustics; Address; Alberta province; Animals; Attention; BRAIN initiative; Back; Blood; Blood flow; Brain; Brain imaging; Calcium; Cells; Collaborations; Development; Devices; Drug Delivery Systems; Electrodes; Elements; Frequencies; Functional Imaging; Functional Magnetic Resonance Imaging; Future; Glass; Head; Image; Imaging Phantoms; Imaging technology; Individual; Lasers; Lead; Light; Lighting; Measures; Methods; Microscopy; Modeling; Molecular; Neurons; Neurosciences; Neurosciences Research; Noise; Optics; Physiologic pulse; Public Health; Rattus; Reporter; Research; Research Methodology; Research Personnel; Resolution; Rodent; Scheme; Signal Transduction; System; Technology; Testing; Text; Time; Transducers; Ultrasonic Transducer; Ultrasonography; United States National Institutes of Health; Universities; Variant; Vibrissae; Work; animal imaging; awake; blood-brain barrier disruption; cellular imaging; cerebral blood volume; design; experimental study; human subject; imaging modality; implantable device; indium tin oxide; neonate; neuroimaging; neurosurgery; next generation; novel; optical imaging; optogenetics; photoacoustic imaging; programs; public health relevance; temporal measurement; translational potential; ultrasound

Project Summary/Abstract Section Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. We propose the development of a novel bias-switchable row-column 2D ultrasound transducer array technology for 3D ultrasound and photoacoustic imaging of whole-brain functional activity with potential for a wearable format for awake and moving animals. These can be developed into high- frequency arrays and even transparent variants that should allow optical and ultrasonic imaging down to resolutions of 30 microns, approaching single-neuron scales. Unlike previous 2D array technologies that require wires connecting to each transducer element, our approach requires only row- and column addressing, yet permits readout from every element of the array using novel electrostrictive bias switching approaches. The technology promises first-of-it’s kind volumetric functional brain imaging of blood-flow changes, blood oxygenation changes, and even neuron activity over the whole brains of awake and moving rodents. The array offers significant potential for next-generation neuroscience experiments involving both optical and acoustic methods, including future work combining functional ultrasound and photoacoustic imaging with optogenetics, optical microscopy of genetically-encoded reporters of neuron activity, ultrasound neuro-stimulation, ultrasound blood-brain barrier disruption for drug delivery, and more. The arrays have considerable translational potential for future human subject imaging in neurosurgeries, neonates, and with potential for implantable devices for longitudinal functional brain imaging. Our research methods include careful design and fabrication of these high-frequency arrays with special attention to transparent variants. We also develop novel imaging schemes and implement these schemes on programmable ultrasound systems. Testing will include array characterization, imaging phantoms, imaging cells expressing near-infrared (NIR) genetically-encoded reporters of neuron Calcium activity. If successful, future work will go on to implement animal imaging experiments aiming to visualize functional brain imaging due to whisker stimulation in both anesthetized and awake- moving animals.

项目摘要/摘要部分 在此输入您的申请的新摘要信息的文本。此部分的文本长度不得超过 30 行。 我们建议开发一种新型偏置可切换行列 2D 超声换能器阵列技术，用于全脑功能活动的 3D 超声和光声成像，并有可能成为清醒和运动动物的可穿戴格式。这些技术可以开发为高频。阵列甚至透明变体，可以将光学和超声成像分辨率降低至 30 微米，这与之前需要将电线连接到每个换能器元件的单神经元尺度不同。我们的方法只需要行和列寻址，但允许使用新颖的电致伸缩偏置切换方法从阵列的每个元素中读出该技术有望实现血流变化、血氧变化和血流变化的体积功能脑成像。该阵列甚至为清醒和运动啮齿类动物整个大脑的神经元活动提供了光学和声学方法的下一代神经科学实验的巨大潜力，包括将功能性超声和光声成像与光遗传学、光学显微镜相结合的未来工作。这些阵列在神经外科、新生儿和遗传领域的未来人体成像方面具有巨大的转化潜力，并且具有用于纵向功能性脑成像的植入设备的潜力。我们的研究方法包括仔细设计和制造这些高频阵列，并特别关注透明变体。我们还开发新颖的成像方案并在可编程超声系统上实施这些方案，测试将包括阵列表征、成像。如果成功的话，未来的工作将继续实施动物成像实验，旨在可视化由于麻醉和清醒状态下的胡须遗传刺激而产生的功能性大脑成像。 - 动物。