Role of hopanoid microbial lipids in a legume:microbe nitrogen-fixing symbiosis

藿香类微生物脂质在豆科植物中的作用：微生物固氮共生

基本信息

批准号：
10470373
负责人：
Brittany Jo Belin
金额：
$ 24.9万
依托单位：
CARNEGIE INSTITUTION OF WASHINGTON, D.C.
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10470373
关键词：
Actins Affect Antibiotic Resistance Antibiotics Award Bacillus cereus Bacteria Bacterial Infections Biological Process Biophysics Biosensor Bradyrhizobium Cell Wall Cell division Cell surface Cells Chemicals Complex Cytoskeleton Defect Development Doctor of Philosophy Environment Eukaryota Exposure to Fabaceae Genetic Determinism Goals Growth High temperature of physical object Hydrocarbons Immune Infection Institution Laboratories Lead Lipids Lipopolysaccharides Measures Mechanics Mediating Mediator of activation protein Membrane Microbe Microbial Physiology Microelectrodes Mitosis Modeling Modification Molecular Profiling Nitrogen Nodule Nuclear Organ Organism Organogenesis Penetration Peptidoglycan Plant Roots Plants Process Quantitative Microscopy Recording of previous events Research Resistance Role S-Phase Fraction Signal Transduction Signaling Molecule Steroids Sterols Stress Surface Survival Rate Symbiosis System Tail Testing Time Tissues Training Training Support Work antimicrobial bacterial resistance beneficial microorganism career chronic infection environmental stressor extracellular gene product human pathogen human tissue microbial mutant pathogen pathogenic bacteria pathogenic microbe physical property pressure research study response skills stressor symbiont tissue culture

项目摘要

Project Abstract It is well known that components of the bacterial cell wall are crucial to successful host interactions for both pathogenic and beneficial microbes. While detailed mechanistic information is available on the roles of a subset of cell wall components (i.e. lipopolysaccharides (LPS) and peptidoglycan) in these associations, few other bacterial cell surface molecules have been as rigorously investigated. Bacterial hopanoids are emerging as new determinants of the efficiency of the interactions between a subset of bacteria and their eukaryotic hosts. Hopanoids are sterol-like lipids produced by diverse eukaryote-associated bacteria, including the human pathogens in the Burkolderia cepacia complex and Bacillus cereus spp. In addition to rigidifying bacterial membranes, similarly to eukaryotic sterols, hopanoids are known to confer broad stress resistance to bacteria in culture. In native infection contexts, the significance of the increase stress resistance provided by hopanoids, and whether hopanoids confer other advantages, has not been mechanistically clarified. Recent work in the Newman lab has demonstrated that a specific class of hopanoids, extended hopanoids containing an extracellular hydrocarbon tail, regulates the efficiency of the symbiotic interaction between the nitrogen-fixing bacterium Bradyrhizobium diazoefficiens with the legume host Aeschynomene afraspera. I have determined that extended hopanoids affect the progression of the symbiosis at all stages, including the initial bacterial infection of plant roots and subsequent proliferation of bacterial symbionts within host cells. I hypothesize that the role of extended hopanoids during these stages is to enhance bacterial growth under abiotic stressors, specifically the mechanical and chemical pressures provided by the host niche. In my Research Plan, I propose to characterize further the role of extended hopanoids in the B. diazoefficiens-A. afraspera symbiosis and to use this system as a model for how hopanoids facilitate persistent infections of eukaryotic hosts more generally. In my PhD I studied the nuclear actin cytoskeleton in human tissue culture models, using approaches in quantitative microscopy and biophysics, and completing my Research Plan will require me to significantly expand my skill set. I am applying for a K99 award to support the training I need to develop both the research and non-research skills necessary to achieve my long-term career goal of establishing a successful independent laboratory at an R1 institution. I hope to synthesize approaches in biophysics, quantitative microscopy, plant cultivation and microbial physiology to study microbial responses to the chemical and mechanical environments of their hosts. I believe that my training history makes me uniquely suited to occupy this under-studied research niche, which I expect to yield key principles of microbial adaptation to hosts that will be broadly applicable to many host:microbe systems.

项目摘要众所周知，细菌细胞壁的成分对于成功宿主至关重要致病微生物和有益微生物之间的相互作用。虽然详细的机械信息是可用于了解细胞壁成分子集（即脂多糖 (LPS) 和肽聚糖）在这些关联中，很少有其他细菌细胞表面分子被如此严格地调查了。细菌藿类化合物正在成为相互作用效率的新决定因素细菌子集与其真核宿主之间的关系。 Hopanoids 是由以下物质产生的类甾醇脂质多种真核相关细菌，包括洋葱伯克氏菌中的人类病原体复合物和蜡状芽孢杆菌属。除了使细菌膜硬化外，与真核生物类似已知甾醇、藿类化合物可赋予培养物中细菌广泛的应激抗性。在原生感染中藿类化合物提供的增加抗压能力的重要性，以及藿类化合物是否赋予其他优势，尚未从机制上阐明。纽曼实验室最近的工作表明，一类特定的藿香类化合物，可扩展含有细胞外碳氢化合物尾部的藿香类化合物，调节共生体的效率固氮细菌重氮慢生根瘤菌与豆科植物宿主之间的相互作用 Aeschynomene afraspera。我已经确定延长的藿类药物会影响病情的进展所有阶段的共生，包括植物根部的初始细菌感染和随后的增殖宿主细胞内的细菌共生体。我假设延长的藿类化合物在这些过程中的作用阶段的目的是增强细菌在非生物应激源下的生长，特别是机械和化学应激宿主生态位提供的压力。在我的研究计划中，我建议进一步描述 B. diazoefficiens-A 中延长的藿类化合物。 afraspera 共生并使用该系统作为模型藿香类化合物如何更普遍地促进真核宿主的持续感染。在我的博士学位中，我研究了人体组织培养模型中的核肌动蛋白细胞骨架，使用定量显微镜和生物物理学的方法，完成我的研究计划将需要我显着扩展我的技能。我正在申请 K99 奖项来支持我需要的培训发展实现我的长期职业目标所需的研究和非研究技能在 R1 机构建立成功的独立实验室。我希望能够综合各种方法生物物理学、定量显微镜、植物栽培和微生物生理学来研究微生物对宿主的化学和机械环境的反应。我相信我的训练历史使我非常适合占据这个尚未得到充分研究的研究领域，我希望它能产生关键的成果微生物适应宿主的原理将广泛适用于许多宿主：微生物系统。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Extended Hopanoid Loss Reduces Bacterial Motility and Surface Attachment and Leads to Heterogeneity in Root Nodule Growth Kinetics in a Bradyrhizobium-Aeschynomene Symbiosis.

长时间的 Hopanoid 损失会降低细菌的活力和表面附着，并导致慢生根瘤菌-Aeschynomene 共生中根瘤生长动力学的异质性。

DOI：
发表时间：
2019-10
期刊：
影响因子：
0
作者：
Belin, Brittany J;Tookmanian, Elise M;de Anda, Jaime;Wong, Gerard C L;Newman, Dianne K
通讯作者：
Newman, Dianne K

The role of hopanoids in fortifying rhizobia against a changing climate.

藿类化合物在强化根瘤菌应对气候变化方面的作用。

DOI：
10.1111/1462-2920.15594
发表时间：
2021-05-14
期刊：
Environmental microbiology
影响因子：
5.1
作者：
Elise Tookmanian;Brittany J Belin;J. Sáenz;D. Newman
通讯作者：
D. Newman

Hopanoid lipids promote soybean- Bradyrhizobium symbiosis.

Hopanoid 脂质促进大豆-慢生根瘤菌共生。

DOI：
发表时间：
2023-09-05
期刊：
影响因子：
0
作者：
Pan, Huiqiao;Shim, Ashley;Lubin, Matthew B;Belin, Brittany J
通讯作者：
Belin, Brittany J

Hopanoid lipids promote soybean-Bradyrhizobium symbiosis.

Hopanoid 脂质促进大豆-慢生根瘤菌共生。

DOI：
发表时间：
2024-04-10
期刊：
影响因子：
6.4
作者：
Pan, Huiqiao;Shim, Ashley;Lubin, Matthew B;Belin, Brittany J
通讯作者：
Belin, Brittany J

Technische Universität München Lehrstuhl für Phytopathologie Lipopolysaccharide of plant-associated bacteria and their role in innate immunity of Arabidopsis thaliana

慕尼黑工业大学植物病理学植物相关细菌的脂多糖及其在拟南芥先天免疫中的作用

DOI：
10.1145/3478431.3499273
发表时间：
2024-09-13
期刊：
Proceedings of the 53rd ACM Technical Symposium on Computer Science Education - Volume 1
影响因子：
0
作者：
C. Dawid;N. Gisch
通讯作者：
N. Gisch