Yale Center for Genome Analysis Shared Resource

耶鲁大学基因组分析中心共享资源

基本信息

批准号：
10461895
负责人：
SHRIKANT M MANE
金额：
$ 28.45万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-07-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10461895
关键词：
Applications Grants Authorship Bioinformatics Biological Assay Biological Sciences Biotechnology Blood specimen Cancer Biology Cancer Center Support Grant Cells Complement Computer software Consultations Custom DNA DNA sequencing Data Data Analyses Doctor of Philosophy Educational workshop Epigenetic Process Equipment Experimental Designs Expression Profiling Funding Gene Expression Genetic Genetic Transcription Genomics Genotype Grant High Performance Computing Institutes Ions Laboratories Large-Scale Sequencing Libraries Linux Malignant Neoplasms Methodology Methods Methylation Mission Persons Preparation Protocols documentation Publications RNA Research Research Personnel Resource Sharing Resources Sampling Scientist Sequence Analysis Services Technology Testing Time Tissues Training Training and Education United States National Institutes of Health Variant Vendor Wages Work Writing Yale Cancer Center cost data quality design experience experimental study genome analysis genomic platform improved instrument interest member new technology next generation next generation sequencing professor research and development secondary analysis sequencing platform square foot tool transcriptome sequencing

项目摘要

YALE CENTER FOR GENOME ANALYSIS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Yale Center for Genome Analysis (YCGA) was established in 2010 to provide next-generation sequencing (NGS), single-cell genomics, and microarray support forYCC members. The Center is a full-service facility dedicated to providing RNA expression profiling, DNA genotyping, and next-Gen DNA sequencing and related state-of-the-art technologies. Full microarray and next-generation sequencing services provided by YCGA include: DNA and RNA isolation from tissue and blood; sample preparation ready for microarray and next-Gen sequence (NGS) analysis; providing primary data; full-time YCC-supported staff for downstream bioinformatics data analysis, working collaboratively with YCC investigators and providing methods and other write-ups for grants. Additionally, the staff has obtained equipment and center grants to reduce the cost of sequencing. During the most recent funding period, 211 people have used YCGA Eighty-seven of these users (41%) were YCC members. The majority of this use came from Genomics, Genetics and Epigenetics (GGE). YCGA provides free consultation and training for YCC members. The core is led by Director Shrikant Mane, PhD, Professor of Genetics, whose experience is complemented by the strong expertise of Associate Director Kaya Bilguvar, MD, PhD. The Specific Aims of YCGA are to provide: (1) Next-generation sequencing services using multiple sequencing platforms, including Illumina, Ion Torrent, and Pacific Biosciences; (2) Microarray services using Affymetrix and Illumina platforms for gene expression, genotyping, and methylation assays; (3) Single cell genomics and structural variation determination using the 10X Genomics platform; (4) Research and development. YCGA continuously carries out biotechnology research to develop improved methodologies and tools, to test the relative merits of new technologies, and to generate preliminary results for NIH grants. (5) Bioinformatics. Dr. Francesc Lopez supports the secondary analysis needs of YCC members. His salary is supported by YCC. (6) Consultation. YCGA makes every effort to develop collaborative relationships with its users, and takes interest in their scientific projects. (7) Education and training. Staff members actively participate in YCC Grand Rounds, departmental seminars, and YSM Dean’s workshops aimed at providing principles and technical details of the various genomic technologies.

耶鲁大学基因组分析共享资源中心项目概要/摘要耶鲁大学基因组分析中心 (YCGA) 成立于 2010 年，提供下一代测序服务该中心为 YCC 会员提供 NGS、单细胞基因组学和微阵列支持。致力于提供 RNA 表达谱、DNA 基因分型和下一代 DNA 测序及相关服务 YCGA 提供最先进的技术。包括：从组织和血液中分离 DNA 和 RNA；为微阵列和下一代做好准备的样品制备序列 (NGS) 分析；为下游生物信息学提供全职 YCC 支持人员数据分析，与 YCC 研究人员合作并提供方法和其他文章此外，工作人员还获得了设备和中心拨款，以降低测序成本。在最近的资助期间，有 211 人使用过 YCGA，其中 87 人 (41%) 是 YCC 成员大部分来自基因组学、遗传学和表观遗传学 (GGE)。 YCGA为YCC会员提供免费咨询和培训，核心由总监Shrikant Mane领导，博士，遗传学教授，副主任的强大专业知识补充了他的经验卡亚·比尔古瓦尔（Kaya Bilguvar），医学博士、哲学博士。 YCGA的具体目标是提供：（1）使用多重测序的下一代测序服务 (2) 使用 Affymetrix 和的微阵列服务用于基因表达、基因分型和甲基化测定的 Illumina 平台；(3) 单细胞基因组学和使用10X Genomics平台进行结构变异测定；（4）YCGA研究和开发。不断开展生物技术研究，开发改进的方法和工具，测试新技术的相对优点，并为 NIH 拨款产生初步结果。 (5) 生物信息学博士。 Francesc Lopez支持YCC成员的二次分析需求，他的薪水由YCC支持（6）。 YCGA 尽一切努力与用户建立合作关系，并对此感兴趣。 (7) 工作人员积极参与 YCC Grand Rounds，部门研讨会和 YSM 院长研讨会旨在提供原则和技术细节各种基因组技术。