Histology Core

组织学核心

基本信息

批准号：
10462727
负责人：
Colin K Combs
金额：
$ 29.62万
依托单位：
UNIVERSITY OF NORTH DAKOTA
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-13 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10462727
关键词：
Applications Grants Biomedical Research Businesses Centers of Research Excellence Cities Communities Core Facility Coupled Drops Ensure Equipment Experimental Designs Fee-for-Service Plans Fees Floor Growth Health Sciences Histological Techniques Histology Human Image Image Analysis Infrastructure Institution Instruction Investments Maintenance Morphology North Dakota Nutritional Study Outcome Pathologist Phase Preparation Productivity Publications Recommendation Research Research Personnel Resolution Resources Safety Sampling Services Slide Stains Structure Students Suggestion Techniques Time Tissue Banks Tissue Fixation Tissue Sample Tissues Training United States Department of Agriculture United States National Institutes of Health Universities Veterinarians Vision analytical tool base cost detection method fluorescence imaging improved instrument instrumentation medical schools meetings member pathogen programs response sample fixation tissue preparation tribal college undergraduate student whole slide imaging

项目摘要

Summary The Histology Core at UND was established to improve the infrastructure supporting our successful implementation of the Phase 1 Center for Excellence in Host Pathogen Interactions COBRE at the University of North Dakota School of Medicine and Health Sciences. The Core was developed to provide high quality instrumentation, tissue preparation, staining, image analysis services, and training in a wide spectrum of fundamental and advanced histological techniques to COBRE research investigators. The Core also provides access and support to non-COBRE investigators within and outside the UND SMHS, as well as those from institutions adjoining (USDA-Grand Forks Human Nutrition Research Center) and in neighboring cities (North Dakota State University, North Dakota primarily undergraduate institutions and tribal colleges). The Core remains essential for successful growth of the host pathogen interaction enterprise at UND and completing al four of the projects proposed in this Phase 2 renewal application. The Core evolved from being a primarily shared equipment facility with provided user training to now a complete drop off service facility for users across the UND campus. Multiple tissue sectioning and staining services coupled with whole slide brightfield or fluorescent imaging and image quantitation have made the Core invaluable to growth of the research enterprise at UND. In Phase 2, the Core will continue to assist COBRE team members and the broader UND/ND research community with experimental design specifically with regards to application-specific sample preparation techniques, identifying appropriate analytical tools available, resolution restrictions, and quantitative morphological techniques. The Core will also provide service, training, and instruction to COBRE and other users in all aspects of instrument use, safety, tissue sample preparation, and aid with trouble shooting all aspects of tissue preparation and analysis. Finally, the Core will implement a business plan that includes strategies to continue expanding the user base and provide fee-for-service assistance in all aspects of histo- and immunochemical detection methods and analyses, thus paving a way to long-term sustainability of the Core.

概括 UND 组织学核心的建立是为了改善支持我们成功的基础设施在大学实施宿主病原体相互作用 COBRE 第一阶段卓越中心北达科他州医学与健康科学学院。核心的开发是为了提供高质量仪器、组织制备、染色、图像分析服务以及广泛的培训为 COBRE 研究人员提供基础和先进的组织学技术。核心还提供接触并支持 UND SMHS 内部和外部的非 COBRE 调查员以及来自毗邻的机构（美国农业部大福克斯人类营养研究中心）和邻近城市（北北达科他州达科他州立大学主要是本科院校和部落学院）。核心对于 UND 宿主病原体相互作用事业的成功发展和完成所有任务仍然至关重要第二阶段续展申请中提出的四个项目。核心是从主要的共享设备设施并提供用户培训，现在为跨地区的用户提供完整的下车服务设施 UND 校园。多种组织切片和染色服务以及整个载玻片明场或荧光成像和图像定量使核心对研究的发展具有无价的价值 UND 的企业。在第二阶段，核心将继续协助 COBRE 团队成员和更广泛的人员 UND/ND 研究社区，专门针对特定应用样本进行实验设计制备技术、确定可用的适当分析工具、分辨率限制和定量形态学技术。该核心还将向 COBRE 和其他机构提供服务、培训和指导用户在仪器使用、安全、组织样本制备以及故障排除等各个方面的帮助组织制备和分析方面。最后，核心将实施一项业务计划，其中包括继续扩大用户基础并在历史的各个方面提供按服务收费的援助的战略和免疫化学检测方法和分析，从而为长期可持续发展铺平道路核。