ANIMAL FACILITY IMPROVEMENT

动物设施改善

• 批准号: 2283658
• 负责人: Teresa Sylvina
• 金额: $ 67.99万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283658
• 关键词: ANIMAL; FACILITY; IMPROVEMENT

Presently, 60 individual research projects which require the use of laboratory animals are conducted by 30 investigators at The Salk Institute. These projects, with annual direct costs totalling $7,913,459, include studies in cancer research; molecular, developmental, and systems neurobiology; endocrinology; developmental biology and immunology. In 1988, a comprehensive effort was initiated by the Institute to better support the needs of existing and projected animal research projects, to better comply with provisions of the Animal Welfare Act and PHS policies related to laboratory animal care and use, and to enable the Institute to become AAALAC accredited. Over the past three years, significant improvements have been made in both the animal facilities and the veterinary care and animal husbandry programs to achieve these objectives. PHS support will be needed, however, for the following necessary alterations and renovations of the Cancer Research Animal Facility (CRAF) and equipment purchases to bring the overall objectives for the Institute's animal research programs to fruition. 1. Ensure effective and routine sanitization and/or sterilization by installing one new cage and rack washer, one new cage and bottle washer, one new floor loading autoclave and one new chlori-flush station to upgrade the existing cagewashing area; installing new bumper guards to protect exposed wall surfaces; and purchasing new portable stainless steel racks for holding rat and mouse cages. 2. Improve air quality and minimize cross contamination by installing a new main exhaust duct and new exhaust dampers, adjusting the pitch of fan blades and rebalancing the air supply and exhaust to achieve an acceptable number of room air changes/hour and correct air flow patterns. 3. Establish a centrally managed surgery area that meets NIH standards and the needs of investigators by providing staffing and equipping a centrally located surgery area for major survival surgery on rats, cats and monkeys. 4. Minimize the potential for introducing pathogens into established animal colonies by providing a quarantine area with two animal cubicles and 1 HEPA-filtered biological safety cabinet that is adjacent to, but physically separate from, other animal housing rooms; and purchasing five additional HEPA-filtered laminar flow workbenches to ensure maintenance of the Viral-Antibody-Free status of transgenic and immunodeficient mice (radiation chimeras, SCID and nu/nu) when changing sterilized microisolator cages and performing minor experimental procedures. 5. Meet NIH and USDA standards for housing cats and monkeys and enhance their psychological well-being by replacing existing caging with new stainless steel cages.

目前，需要使用的60个个人研究项目 Salk Institute的30名研究人员进行了实验动物。 这些项目，年度直接成本总计7,913,459美元，包括 癌症研究的研究；分子，发育和系统 神经生物学；内分泌学；发育生物学和免疫学。 在 1988年，研究所发起了一项全面的努力，以改善 支持现有和预测的动物研究项目的需求， 更好地遵守《动物福利法》和PHS政策的规定 与实验室动物护理和使用有关，并使研究所能够 成为AAALAC认可的。 在过去的三年中，很重要 动物设施和 兽医护理和畜牧业计划，以实现这些目标。 但是，对于以下必要的需要，需要PHS支持 癌症研究动物设施（CRAF）的改变和翻新 购买设备以实现该研究所的总体目标 动物研究计划。 1.通过 安装一个新的笼子和架子洗衣机，一个新的笼子和瓶洗衣机， 一个新的地板装载高压灭菌器和一个新的氯粉量升级站来升级 现有的笼子清洗区；安装新的保险杠保护以保护 裸露的墙面；并购买新的便携式不锈钢架 用于握住老鼠和鼠标笼。 2。提高空气质量并通过安装一个 新的主排气管和新的排气阻尼器，调整风扇的音高 刀片和重新平衡空气供应和排气以实现可接受的 房间的空气变化/小时和正确的气流模式。 3。建立一个符合NIH标准的中央管理的手术区域 调查人员的需求通过提供人员和中心配备 位于大鼠，猫和猴子的大型生存手术的手术区域。 4。最大程度地减少将病原体引入已建立的潜力 动物殖民地通过提供一个带有两个动物隔间的隔离区和 1与HEPA过滤的生物安全柜相邻，但 与其他动物住房室的物理分开；并购买五个 额外的HEPA过滤层流工作台，以确保维护 转基因和免疫缺陷小鼠的无病毒抗体状态 （辐射嵌合体，SCID和NU/NU）在更换灭菌微分机时 笼子和执行次要的实验程序。 5。符合NIH和USDA住房猫和猴子的标准并增强 他们的心理健康是通过用新的笼子代替现有的笼子 不锈钢笼。