COMPUTER ATLAS OF MOUSE DEVELOPMENT

鼠标发育的计算机图谱

• 批准号: 2202875
• 负责人: BETSEY S WILLIAMS
• 金额: $ 43.29万
• 依托单位: MURITECH
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2202875
• 关键词: complementary RNA; developmental genetics; embryogenesis; gene expression; genetically modified animals; in situ hybridization; information systems; laboratory mouse; mammalian embryology; nucleic acid probes; online computer

The object of this research is to expand upon the progress made in Phase l to create a set of prototype computer tools and services for scientists in the biotechnology and pharmaceutical industries, and in academia, to use for the assessment and analysis of normal and abnormal mouse embryonic development. These tools will take the form of Internet-accessible two and three-dimensional computer-based color atlases of normal mouse development, ages d6-18; associated researcher tools, such as an online dictionary of mouse development and a context-sensitive transparent "doorway" into the Medline database; a morphological map of gene expression; as well as a consulting service for expert assessment of individual cases. The products of this project will expedite research and advance knowledge in many fields, especially in the fields of molecular biology, genetic engineering, developmental biology and teratology. This system will provide widespread access to its resources by using the World Wide Web for its underlying architecture. We will expand upon our already-proven interactive-remote-visualization (IRV) server technology to develop a highly-scalable model of a dedicated Internet visualization server which will provide interactive control of real-time visualizations from the Muritech database. These will be embedded within World Wide Web multimedia documents using our proprietary Mosaic-OLE technology. We will create an object-oriented knowledge base of embryonic anatomy, with voxel- level object indexing and semantic representations of anatomical hierarchies and relationships. We will expand the semantic tree to include relevant functional and genetic information, and will use this as a basis for facilitated navigation among the various databases of the Muritech system. Gateways will be included to external databases, such as the Visible Embryo Project and the Mouse Genome Project. We will use the database and software tools that we develop to formulate commercial prototypes of a set of tools and services including, in part, an online database and knowledge base, an online consulting service a microcomputer-based CD ROM atlas. Later phases of this project will establish the full, Internet-accessible, mouse development database, and also establish a consulting service for the assessment of mouse dysmorphologies. PROPOSED COMMERCIAL APPLICATION: The overall aim of this endeavor (Phase I, Phase II and beyond) is to develop products that will provide to the biomedical researcher and to the biotechnology and pharmaceutical industry a means of analyzing and assessing developmental defects in the mouse. The tools and commercial services that we will produce include: 1) an online database and set of visualization tools for client researchers using the World Wide Web and mosaic on the Internet, 2) a microcomputer-based 2- & 3-D CD ROM atlas and knowledge base of normal mouse development and 3) an online consulting service providing case-by-case assessments of morphological defects.

本研究的目的是扩展阶段所取得的进展 l 为科学家创建一套原型计算机工具和服务 在生物技术和制药行业以及学术界， 用于评估和分析正常和异常小鼠胚胎 发展。这些工具将采用可通过互联网访问的两种形式 正常小鼠三维计算机彩色图谱 发育，年龄 d6-18；相关的研究人员工具，例如在线 鼠标发育字典和上下文相关的透明字典 进入 Medline 数据库的“门户”；基因形态图 表达;以及专家评估的咨询服务 个别案例。该项目的产品将加速研究和 推进许多领域的知识，特别是分子领域的知识 生物学、基因工程、发育生物学和畸胎学。 该系统将通过使用 万维网的底层架构。我们将扩大我们的 已经经过验证的交互式远程可视化 (IRV) 服务器技术 开发专用互联网可视化的高度可扩展模型 服务器将提供实时可视化的交互式控制 来自 Muritech 数据库。这些将嵌入万维网中 使用我们专有的 Mosaic-OLE 技术的多媒体文档。我们将 创建一个面向对象的胚胎解剖学知识库，具有体素 解剖学的层次对象索引和语义表示 层次结构和关系。我们将扩展语义树以包括 相关的功能和遗传信息，并将以此为基础 方便在 Muritech 的各个数据库之间导航 系统。网关将包含到外部数据库，例如 可见胚胎计划和小鼠基因组计划。 我们将使用我们开发的数据库和软件工具来制定 一套工具和服务的商业原型，部分包括： 在线数据库和知识库、在线咨询服务 基于微机的CD ROM图集。该项目的后期阶段将 建立完整的、可通过互联网访问的鼠标开发数据库，​​以及 还建立了鼠标评估咨询服务 畸形。 拟议的商业应用：这项工作的总体目标（阶段 I、第二阶段及以后）是开发将提供给 生物医学研究员以及生物技术和制药行业 分析和评估小鼠发育缺陷的方法。这 我们将提供的工具和商业服务包括：1）在线 数据库和一套可视化工具，供客户研究人员使用 万维网和互联网上的马赛克，2）基于微计算机的2-＆ 3-D CD ROM 图集和正常小鼠发育的知识库以及 3) 在线咨询服务提供个案评估 形态缺陷。