MOLECULAR ANALYSIS OF RETROVIRUSES AND ONCOGENES

逆转录病毒和癌基因的分子分析

• 批准号: 2089977
• 负责人: DON GANEM
• 金额: $ 39.34万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-08-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2089977
• 关键词: 3T3 cells; RNA directed DNA polymerase; Retroviridae; biological signal transduction; chickens; frameshift mutation; genetically modified animals; laboratory mouse; laboratory rat; neoplastic transformation; oncogenes; protooncogene; provirus; receptor; ribosomes; tissue /cell culture; transfection; viral carcinogenesis; virus envelope; virus genetics; virus protein; virus replication

A variety of biochemical and genetic techniques will be used to study the replication of retroviruses and some of the genes implicated in retroviral oncogenesis. In the studies on viral replication, emphasis will be placed upon receptors that mediate virus entry, the mechanism of proviral integration, synthesis of viral proteins via ribosomal frameshifting, and assembly of viral envelopes. Two classes of proto-oncogenes, the src and Wnt gene families, will be examined in efforts to understand the role of their protein products in signalling in individual cells, development in whole organisms, transformation of cultured cells, and multi-step oncogenesis in transgenic animals.

各种生化和遗传技术将用于 研究逆转录病毒和某些基因的复制 与逆转录病毒肿瘤发生有关。 在病毒研究中 复制，重点将放在介导的受体上 病毒进入，病毒整合的机制，合成 病毒蛋白通过核糖体框架和病毒组装 信封。 两类的原始基因，SRC和Wnt基因 家庭将进行检查，以了解 它们的蛋白质产物在单个细胞中的信号传导中， 整个生物体的发展，培养细胞的转化， 以及转基因动物的多步肿瘤发生。

项目成果

The src protein contains multiple domains for specific attachment to membranes.

src 蛋白包含多个用于特异性附着到膜上的结构域。

• DOI: 10.1128/mcb.10.3.1000-1009.1990
• 发表时间: 1990
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Kaplan,JM;Varmus,HE;Bishop,JM
• 通讯作者: Bishop,JM

Activity of Wnt-1 as a transmembrane protein.

Wnt-1 作为跨膜蛋白的活性。

• DOI: 10.1101/gad.7.11.2181
• 发表时间: 1993
• 期刊: Genes & development
• 影响因子: 10.5
• 作者: Parkin,NT;Kitajewski,J;Varmus,HE
• 通讯作者: Varmus,HE

Mouse mammary tumors express elevated levels of RNA encoding the murine homology of SKY, a putative receptor tyrosine kinase.

小鼠乳腺肿瘤表达的 RNA 水平升高，该 RNA 编码 SKY（一种假定的受体酪氨酸激酶）的小鼠同源性。

• DOI: 10.1074/jbc.270.12.6872
• 发表时间: 1995
• 期刊: The Journal of biological chemistry
• 作者: Taylor,IC;Roy,S;Yaswen,P;Stampfer,MR;Varmus,HE
• 通讯作者: Varmus,HE

Identification of genes from pattern formation, tyrosine kinase, and potassium channel families by DNA amplification.

通过 DNA 扩增鉴定模式形成、酪氨酸激酶和钾通道家族的基因。

• DOI: 10.1073/pnas.86.12.4372
• 发表时间: 1989
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Kamb,A;Weir,M;Rudy,B;Varmus,H;Kenyon,C
• 通讯作者: Kenyon,C

Highly conserved amino acids in the SH2 and catalytic domains of v-src are altered in naturally occurring, transformation-defective alleles.

SH2 和 v-src 催化结构域中高度保守的氨基酸在天然存在的转化缺陷等位基因中发生改变。

• 发表时间: 1994
• 期刊: Oncogene
• 影响因子: 8
• 作者: Verderame,MF;Varmus,HE
• 通讯作者: Varmus,HE