MHC Genetic Typing Core

MHC 基因分型核心

• 批准号: 10440879
• 负责人: Eric J. Vallender
• 金额: $ 7.92万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10440879
• 关键词: AIDS/HIV problem; Acceleration; Acquired Immunodeficiency Syndrome; Alleles; Animal Husbandry; Animals; Behavioral Genetics; Breeding; Budgets; Collaborations; Communities; DNA Library; Data; Databases; Development; Disease Progression; Ensure; Funding; Future; Genetic; Genetic Services; Genetic Variation; Genome; Genomics; Genotype; Geographic Locations; Geography; Goals; Grant; Haplotypes; Health; Immune response; Inbreeding; Infection; Informatics; Institution; MHC Class I Genes; Macaca mulatta; Maintenance; Mind; Monitor; Other Genetics; Output; Population; Predisposition; Preventive Medicine; Primates; Process; Protocols documentation; Research; Research Activity; Research Personnel; Role; SIV; Scientist; Services; Source; Structure; System; Targeted Research; Testing; Time; Translational Research; Wisconsin; animal colony; animal data; base; data sharing; deep sequencing; design; evidence base; exhaustion; exome sequencing; experience; genetic information; genetic pedigree; genetic resource; genetic testing; genome sequencing; genomic data; genomic variation; improved; male; nonhuman primate; programs; reproductive; research study; simian human immunodeficiency virus; testing services; tool; whole genome; working group

Project Summary/Abstract - MHC Genetic Typing Core The SPF rhesus macaque breeding colony for AIDS research at the Tulane National Primate Research Center (TNPRC) is part of an extensive evidence-based, integrative, program that incorporates best practices in health surveillance, husbandry, preventive medicine, and behavioral and genetic management. The goals of the genetic management program are to 1) maintain genetic diversity and minimize inbreeding to support sustainable populations and 2) characterize class I MHC alleles in support of HIV/AIDS research activities. To this end, the TNPRC Genome Bank collects and stores materials from more than 9000 unique animals, with additional animals collected as they are produced. The colony is characterized for ancestral geographic-origin and parentage confirmed using a pan-NPRC SNP panel. MHC typing for all animals includes testing for 10 class I alleles, which serves the needs of most core and affiliate scientists, and MHC deep sequencing in collaboration with the Wisconsin National Primate Research Center for targeted research programs. Over the previous grant funding period whole genome sequencing (WGS) has been added for 164 key breeders, focusing on those males with the greatest reproductive output in the breeding colony, and whole exome sequencing (WES) from 72 additional animals. Support has also been obtained for WGS on an additional 200 animals during the budget period for this proposal. These efforts are supported by core TNPRC scientists in collaboration with researchers from other NPRCs and research institutions. These efforts are made in concert with the broader NPRC community through consortia activities, notably, the Nonhuman Primate Genetics and Genomics Working Group. Presently, the efforts of the genetic management program and MHC Genetic Typing Core are to continue to provide high-quality MHC characterization of the SPF breeding colony in support of HIV/AIDS research, to integrate MHC typing with other genetic and genomic data currently being characterized at the TNPRC, and to develop additional support structures for sharing this data with the research community. These efforts ensure the SPF rhesus macaque breeding colony is healthy, sustainable, and of maximal translational research value to the broader HIV/AIDS research community.

项目摘要/摘要 - MHC 基因分型核心 杜兰国家灵长类动物研究中心用于艾滋病研究的 SPF 恒河猴繁殖群 (TNPRC) 是一个广泛的循证综合计划的一部分，该计划纳入了健康方面的最佳实践 监测、畜牧业、预防医学以及行为和遗传管理。遗传的目标 管理计划是 1) 保持遗传多样性并尽量减少近亲繁殖以支持可持续发展 人群和 2) 表征 I 类 MHC 等位基因以支持 HIV/艾滋病研究活动。为此， TNPRC 基因组库收集并存储来自 9000 多种独特动物的材料，以及额外的材料 动物在生产时就被收集。该殖民地的特点是祖先的地理起源和 使用泛 NPRC SNP 面板确认亲子关系。所有动物的 MHC 分型包括 10 种 I 类测试 等位基因，满足大多数核心和附属科学家的需求，并与 MHC 深度测序合作 与威斯康星国家灵长类动物研究中心合作开展有针对性的研究项目。超过上一次补助金 为 164 个关键种鸡增加了资助期全基因组测序 (WGS)，重点关注那些雄性 在繁殖群体中具有最高的繁殖产量，并从 72 进行全外显子组测序 (WES) 额外的动物。预算期间还获得了对另外 200 只动物进行全基因组测序 (WGS) 的支持 本提案的期限。这些努力得到了 TNPRC 核心科学家与研究人员合作的支持 来自其他 NPRC 和研究机构。这些努力是与更广泛的 NPRC 协调一致进行的 社区通过联盟活动，特别是非人类灵长类动物遗传学和基因组学工作组。 目前，遗传管理计划和MHC基因分型核心的努力正在继续 提供 SPF 繁殖群体的高质量 MHC 特征，以支持 HIV/AIDS 研究， 将 MHC 分型与 TNPRC 目前正在表征的其他遗传和基因组数据相结合，并 制定额外的支持结构，以便与研究界共享这些数据。这些努力确保 SPF级恒河猴繁殖群体健康、可持续，具有最大的转化研究价值 更广泛的艾滋病毒/艾滋病研究界。