The role of GHS-R in macrophage reprogramming during meta-inflammation

GHS-R 在元炎症期间巨噬细胞重编程中的作用

• 批准号: 10431889
• 负责人: YUXIANG SUN
• 金额: $ 31.23万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431889
• 关键词: Ablation; Adipocytes; Adipose tissue; Age; Aging; Anti-Inflammatory Agents; Attenuated; Binding; Biology; Bone Marrow; CD14 Antigen; Cell physiology; Cells; Chronic; Coculture Techniques; Cues; Data; Deposition; Diet; Endocrine; Endotoxins; Exhibits; G-Protein-Coupled Receptors; GTP-Binding Proteins; Genus Hippocampus; Glycolysis; Goals; Hepatic; Hepatocyte; High Fat Diet; Hormones; Immunomodulators; Inflammation; Inflammatory; Insulin Resistance; Knock-out; Kupffer Cells; Light; Link; Lipids; Lipopolysaccharides; Liver; Liver Dysfunction; Mass Spectrum Analysis; Mediating; Metabolic; Metabolic Diseases; Metabolic dysfunction; Metabolism; Molecular; Morphology; Mus; Myelogenous; Obesity; Palmitates; Peripheral; Phenotype; Phosphoproteins; Process; Protein Subunits; Reporting; Role; Saturated Fatty Acids; Series; Signal Pathway; Signal Transduction; Steatohepatitis; Thinness; Tissues; antagonist; cytokine; detection of nutrient; diet-induced obesity; dietary; extracellular; fatty acid oxidation; feeding; gain of function; ghrelin; glucose uptake; growth hormone secretagogue receptor; in vivo; insulin sensitivity; insulin signaling; knock-down; loss of function; macrophage; novel; obesity treatment; obesogenic; overexpression; paracrine; response; transcriptome sequencing; translational potential

Obesity promotes chronic inflammation in peripheral tissues such as adipose tissue and liver, which leads to a wide range of metabolic dysfunctions, a phenomenon called “meta-inflammation”. Macrophages are key immune-modulators of meta-inflammation, comprised of pro-inflammatory M1 and anti-inflammatory M2. Reprograming macrophage polarization is thought to have great potential for treatment of obesity-induced inflammation and metabolic dysfunctions. However, the regulatory mechanisms of macrophage polarization are not well understood. Growth hormone secretagogue receptor (GHS-R), is known to bind to nutrient-sensing obesogenic gut hormone ghrelin. Our studies with GHS-R global deletion mice have shown that GHS-R is an essential metabolic regulator; its signaling activation is indicative of systemic metabolic and inflammatory state. We reported that GHS-R ablation protects against diet-induced obesity and insulin resistance in aging. We also have evidence that GHS-R has cell-autonomous effect in macrophages, and knockdown of GHS-R shifts macrophages toward M2 spectrum. We hypothesize that GHS-R is a key regulator of macrophage polarization; GHS-R reprograms macrophages toward pro-inflammatory state under diet-induced obesity, leading to inflammation and lipid accumulation in adipose tissue and liver. We will unravel the roles and pertinent mechanisms of GHS-R in macrophage polarization using our newly-generated myeloid-specific GHS-R knockout and re-expression mice. The following comprehensive and complementary Specific Aims will be conducted: Aim 1. Determine whether macrophage GHS-R promotes pro-inflammatory polarization of macrophages under diet-induced obesity, increasing inflammation and lipid deposition in adipose tissue and liver (In vivo studies). Aim 2. Examine whether GHS-R controls macrophage polarization via cell-autonomous action, and promotes inflammation in adipocytes and hepatocytes via paracrine action (Ex vivo studies). Aim 3. Investigate molecular mechanisms by which GHS-R regulates macrophage polarization. We anticipate that GHS-R has a crucial role in macrophage polarization and meta-inflammation in adipose tissues and liver. Specifically, we predict that GHS-R, via insulin signaling, reprograms signaling pathways of fatty acid oxidation and glycolysis, in turn regulating the expression of pro-inflammatory cytokines. This proposal will shed light on a new paradigm for regulating macrophage phenotypic switch, and likely uncover a novel regulatory mechanism linking nutrient sensing, inflammation and metabolism. This proposal will also provide “proof-of-concept” evidence whether targeting GHS-R in macrophages would be an unique and powerful strategy for combating obesity and inflammation.

肥胖会促进脂肪组织和肝脏等周围组织的慢性炎症，从而导致广泛的代谢功能障碍，这种现象称为“元炎症”，巨噬细胞是元炎症的关键免疫调节剂，由促炎性 M1 组成。重编程巨噬细胞极化被认为在治疗肥胖引起的炎症和代谢功能障碍方面具有巨大潜力，但巨噬细胞极化的调节机制尚不清楚。我们对 GHS-R 整体缺失小鼠的研究表明，生长激素促分泌素受体 (GHS-R) 与营养敏感的肥胖肠道激素 ghrelin 结合，其信号激活是一种重要的代谢调节剂。我们报道了 GHS-R 消除可以预防饮食引起的肥胖和衰老过程中的胰岛素抵抗。我们发现 GHS-R 是巨噬细胞极化的关键调节因子；GHS-R 在饮食诱导的肥胖下将巨噬细胞重新编程为促炎症状态，从而导致炎症和脂质积累。我们将利用我们新产生的骨髓特异性 GHS-R 敲除和重新表达来揭示 GHS-R 在巨噬细胞极化中的作用和相关机制。将进行以下全面和补充的具体目标： 目标 1. 确定巨噬细胞 GHS-R 在饮食诱导的肥胖下是否促进巨噬细胞的促炎极化，增加脂肪组织和肝脏中的炎症和脂质沉积（体内研究）目标 2. 检查 GHS-R 是否通过细胞自主作用控制巨噬细胞极化，并通过旁分泌作用促进脂肪细胞和肝细胞炎症（体外研究）。 3. 研究 GHS-R 调节巨噬细胞极化的分子机制 我们预计 GHS-R 在脂肪组织和肝脏的巨噬细胞极化和元炎症中起着至关重要的作用。重新编程脂肪酸氧化和糖酵解的信号通路，进而调节促炎细胞因子的表达，该提议将揭示调节巨噬细胞表型转换的新范例。可能会发现一种将营养传感、炎症和代谢联系起来的新调控机制。该提案还将提供“概念验证”证据，证明针对巨噬细胞中的 GHS-R 是否是对抗肥胖和炎症的独特而强大的策略。

项目成果

GHS-R in brown fat potentiates differential thermogenic responses under metabolic and thermal stresses.

棕色脂肪中的 GHS-R 会增强代谢和热应激下的差异生热反应。

• 发表时间: 2021
• 影响因子: 3.7
• 作者: Lee, Jong Han;Lin, Ligen;Ye, Xiangcang;Wolfrum, Christian;Chen, Yingjie;Guo, Shaodong;Sun, Yuxiang
• 通讯作者: Sun, Yuxiang

Age-dependent remodeling of gut microbiome and host serum metabolome in mice.

小鼠肠道微生物组和宿主血清代谢组的年龄依赖性重塑。

• 发表时间: 2021
• 作者: Wu, Chia;Muthyala, Sai Deepak Venkata;Klemashevich, Cory;Ufondu, Arinzechukwu Uchenna;Menon, Rani;Chen, Zheng;Devaraj, Sridevi;Jayaraman, Arul;Sun, Yuxiang
• 通讯作者: Sun, Yuxiang

Nutrient-sensing growth hormone secretagogue receptor in macrophage programming and meta-inflammation.

巨噬细胞编程和元炎症中的营养感应生长激素促分泌素受体。

• 发表时间: 2024-01
• 影响因子: 8.1
• 作者: Kim, Da Mi;Lee, Jong Han;Pan, Quan;Han, Hye Won;Shen, Zheng;Eshghjoo, Sahar;Wu, Chia;Yang, Wanbao;Noh, Ji Yeon;Threadgill, David W;Guo, Shaodong;Wright, Gus;Alaniz, Robert;Sun, Yuxiang
• 通讯作者: Sun, Yuxiang

Diverse and Complementary Effects of Ghrelin and Obestatin.

生长素释放肽和肥胖抑制素的多样化和互补作用。

• 发表时间: 2022-03-29
• 影响因子: 5.5
• 作者: Villarreal, Daniel;Pradhan, Geetali;Zhou, Yu;Xue, Bingzhong;Sun, Yuxiang
• 通讯作者: Sun, Yuxiang

Neuronal ablation of GHSR mitigates diet-induced depression and memory impairment via AMPK-autophagy signaling-mediated inflammation.

GHSR 的神经元消融通过 AMPK 自噬信号介导的炎症减轻饮食引起的抑郁和记忆障碍。

• 发表时间: 2024
• 作者: Wang, Hongying;Shen, Zheng;Wu, Chia;Ji, Pengfei;Noh, Ji Yeon;Geoffroy, Cédric G;Kim, Sunja;Threadgill, David;Li, Jianrong;Zhou, Yu;Xiao, Xiaoqiu;Zheng, Hui;Sun, Yuxiang
• 通讯作者: Sun, Yuxiang