Shared Resource - Microscopy & Imaging Shared Resource

共享资源 - 显微镜

基本信息

批准号：
10400651
负责人：
MICHAEL D JOHNSON
金额：
$ 5.88万
依托单位：
GEORGETOWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-15 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10400651
关键词：
3-Dimensional Aging Animals Apoptosis Biological Assay Brightfield Microscopy Cancer Center Support Grant Cancer Control Cell surface Cells Code Color Comprehensive Cancer Center Computer software Confocal Microscopy Consultations Cultured Cells Cytogenetics Development Doctor of Philosophy Educational workshop Electron Microscope Electron Microscopy Embryo Experimental Designs Fluorescein-5-isothiocyanate Fluorescence Fluorescence Microscopy Fluorescence Recovery After Photobleaching Fluorescence Resonance Energy Transfer Glass Goals Hybrids Image Image Analysis In Situ Individual Inflammation Injections Interference Reflection Microscopy Investigational Therapies Laboratories Lasers Licensing Methods Microinjections Microscope Microscopy Microtus Mitosis Molecular Cytogenetics Mus NCI Center for Cancer Research Optics Organ Phase Photobleaching Reagent Research Research Personnel Resource Sharing Scanning Services Site Slide Speed Supervision System Three-Dimensional Image Time Tissue imaging Tissues Training Transmission Electron Microscopy Universities angiogenesis anticancer research assay development base beam-splitter cancer prevention cellular imaging charge coupled device camera confocal imaging contrast imaging culture plates design detection sensitivity digital exosome experimental study fluorescence lifetime imaging fluorescence microscope imaging modality imaging system instrument instrumentation lectures light microscopy malignant breast neoplasm member microscopic imaging migration molecular oncology multiphoton imaging photon-counting detector programs red fluorescent protein tissue culture tissue/cell culture transmission process tumor tumorigenesis

项目摘要

The Microscopy & Imaging Shared Resource (MISR) was established in 1989 to provide 1) access to an array of well-maintained instrumentation for Georgetown Lombardi Comprehensive Cancer Center (LCCC) investigators wishing to apply light microscopy and image analysis to cancer research and 2) the expertise, training and reagents necessary for investigators to make optimal use of the instruments available. The MISR provides instrumentation for bright-field microscopy, routine fluorescence microscopy and more advanced applications, including confocal microscopy, microinjection, total internal reflection microscopy (TIRF), multispectral emission imaging, fluorescence lifetime imaging, Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and deep tissue multiphoton imaging. Live cell, time-lapse imaging is possible using five different wide-field and confocal systems. Using MetaMorph v7.8.13 offline image analysis and Volicity v6.3.0 3D Image Analysis Software, MISR develops automated routines for users carrying out common assays, such as cell tracking, proliferation, studies of mitosis and apoptosis plus angiogenesis experiments. The MISR also supports cytogenetics and molecular cytogenetics services and provides the following: • Access to nine major digital microscope workstations, transmission electron microscope and stand-alone image analysis workstations. It also supports 16 image analysis software licenses in individual laboratories. • Expert advice, training and assistance with sophisticated microscopy and image analysis approaches in cancer research. Since the last submission, the MISR 1) replaced an aging confocal system with a new highly capable Leica SP8 AOBS laser scanning confocal microscope, 2) upgraded the TIRF system with a new camera and a coded stage that extends the imaging modalities available and 3) currently supports multiple high-impact studies demonstrating the interaction between distinct cell surface molecules in tumorigenesis and inflammation. The MISR Director, M. Johnson, PhD, leads a team with a wealth of expertise in light and electron microscopy plus image analysis. In 2017, 20 LCCC members representing all four Research Programs (Breast Cancer [BC], Cancer Prevention and Control [CPC], Experimental Therapeutics [ET], and Molecular Oncology [MO]) used the MISR.

显微镜与成像共享资源 (MISR) 成立于 1989 年，旨在提供 1) 对一系列为乔治城隆巴迪综合癌症中心 (LCCC) 研究人员提供维护良好的仪器希望将光学显微镜和图像分析应用于癌症研究以及 2) 专业知识、培训和试剂 MISR 提供了研究人员充分利用可用仪器所必需的仪器。用于明视野显微镜、常规荧光显微镜和更先进的应用，包括共焦显微镜、显微注射、全内反射显微镜 (TIRF)、多光谱发射成像、荧光寿命成像、福斯特共振能量转移 (FRET)、荧光恢复光漂白（FRAP）和深层组织多光子成像可以使用五个活细胞延时成像。使用 MetaMorph v7.8.13 离线图像分析和 Volicity v6.3.0 3D。图像分析软件 MISR 为用户开发自动化程序来执行常见分析，例如细胞分析 MISR 还支持跟踪、增殖、有丝分裂和细胞凋亡研究以及血管生成实验。细胞遗传学和分子细胞遗传学服务并提供以下服务： • 可使用九个主要数字显微镜工作站、透射电子显微镜和独立设备它还支持各个实验室的 16 个图像分析软件许可证。 • 在复杂的显微镜和图像分析方法方面的专家建议、培训和帮助癌症研究。自上次提交以来，MISR 1) 用新型高性能 Leica SP8 取代了老化的共焦系统 AOBS 激光扫描共焦显微镜，2) 使用新相机和编码台升级了 TIRF 系统扩展了可用的成像模式，3) 目前支持多项高影响力研究证明肿瘤发生和炎症中不同细胞表面分子之间的相互作用。 MISR 主任 M. Johnson 博士领导的团队在光学和电子显微镜方面拥有丰富的专业知识 2017 年，20 名 LCCC 成员代表所有四个研究项目（乳腺癌 [BC]、癌症预防和控制 [CPC]、实验治疗学 [ET] 和分子肿瘤学 [MO]）使用米斯尔。